Singhal S, Powles R, Kulkarni S, Treleaven J, Sirohi B, Millar B, Shepherd V, Saso R, Rowland A, Long S, Cabral S, Horton C, Mehta J
Leukaemia Unit, Royal Marsden Hospital, Surrey, UK.
Bone Marrow Transplant. 2000 Mar;25(5):501-5. doi: 10.1038/sj.bmt.1702173.
Forty healthy adult donors underwent marrow (BM) as well as peripheral blood (PBSC) stem cell collections for their HLA-identical adult siblings with hematologic malignancies. BM was harvested on day 1 (target 3 x 108 nucleated cells/kg, 10 microg/kg lenograstim (glycosylated G-CSF) administered on days 2-6, and a single leukapheresis performed on day 6. The blood volume processed was the higher of 200% donor blood volume or 10 liters. The total nucleated cell (TNC) yields from PBSC were 1.1- to 4.3-fold higher than BM (median 7.0 vs 3.1 x 10(8)/kg, P < 0.0001). Although BM contained a higher proportion of CD34+cells (1.3% vs 0.7%, P < 0. 0001) and a comparable proportion of CD3+ cells (median 29% vs 26%, P = 0.4), the absolute numbers of CD34+ and CD3+ cells and their subsets were several times higher in PBSC. There was a poor correlation between BM and PBSC CD34 and TNC numbers, but a significant correlation between BM and PBSC CD3 numbers. Only five of 40 BM harvests contained >/=2 x 10(6) CD34+ cells/kg compared with 35 of 40 PBSC harvests (P < 0.0001). We conclude that the numbers of progenitor and immunocompetent cells in PBSC are several times higher than in BM. It is possible to collect adequate numbers of progenitor cells from blood after lenograstim stimulation more frequently than from marrow, and donors yielding low quantities of progenitor cells from BM usually deliver better quantities from PBSC. Bone Marrow Transplantation (2000) 25, 501-505.
40名健康成年供者为其患有血液系统恶性肿瘤且HLA配型相同的成年同胞进行了骨髓（BM）及外周血（PBSC）干细胞采集。第1天采集骨髓（目标为3×10⁸有核细胞/kg），第2 - 6天给予10μg/kg来格司亭（糖基化G - CSF），并于第6天进行1次白细胞单采。处理的血量为供者血量的200%或10升中的较高值。PBSC的总有核细胞（TNC）产量比BM高1.1至4.3倍（中位数分别为7.0与3.1×10⁸/kg，P < 0.0001）。虽然BM中CD34⁺细胞比例更高（1.3%对0.7%，P < 0.0001）且CD3⁺细胞比例相当（中位数分别为29%对26%，P = 0.4），但PBSC中CD34⁺和CD3⁺细胞及其亚群的绝对数量要高几倍。BM与PBSC的CD34及TNC数量之间相关性较差，但BM与PBSC的CD3数量之间存在显著相关性。40次BM采集中只有5次每千克含有≥2×10⁶个CD34⁺细胞，而40次PBSC采集中有35次（P < 0.0001）。我们得出结论，PBSC中祖细胞和免疫活性细胞的数量比BM高几倍。来格司亭刺激后从血液中比从骨髓中更频繁地采集到足够数量的祖细胞是可能的，而且从BM中产生祖细胞数量少的供者通常从PBSC中能提供更好数量的祖细胞。《骨髓移植》（2000年）25卷，501 - 505页 。
