通过单链可变抗体-载脂蛋白嵌合体靶向纳米盘。
Targeting nanodisks via a single chain variable antibody--apolipoprotein chimera.
作者信息
Iovannisci David M, Beckstead Jennifer A, Ryan Robert O
机构信息
Children's Hospital Oakland Research Institute, 5700 Martin Luther King Jr. Way, Oakland, CA 94609, USA.
出版信息
Biochem Biophys Res Commun. 2009 Feb 6;379(2):466-9. doi: 10.1016/j.bbrc.2008.12.077. Epub 2008 Dec 27.
Nanodisks (ND) are nanometer scale complexes of phospholipid and apolipoprotein that have been shown to function as drug delivery vehicles. ND harboring significant quantities of the antifungal agent, amphotericin B, or the bioactive isoprenoid, all trans retinoic acid, have been generated and characterized. As currently formulated, ND possess limited targeting capability. In this study, we constructed a single chain variable antibody (scFv).apolipoprotein chimera and assessed the ability of this fusion protein to form ND and recognize the antigen to which the scFv is directed. Data obtained revealed that alpha-vimentin scFv.apolipoprotein A-I is functional in ND formation and antigen recognition, opening the door to the use of such chimeras in targeting drug-enriched ND to specific tissues.
纳米盘（ND）是磷脂和载脂蛋白的纳米级复合物，已被证明可作为药物递送载体。已制备并表征了含有大量抗真菌剂两性霉素B或生物活性类异戊二烯全反式维甲酸的纳米盘。按照目前的配方，纳米盘的靶向能力有限。在本研究中，我们构建了一种单链可变抗体（scFv）-载脂蛋白嵌合体，并评估了这种融合蛋白形成纳米盘以及识别scFv所针对抗原的能力。所获得的数据表明，α-波形蛋白scFv-载脂蛋白A-I在纳米盘形成和抗原识别方面具有功能，为将此类嵌合体用于将富含药物的纳米盘靶向特定组织打开了大门。
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