Lee Ming-Min, Sicard Mathieu, Skeie Marjorie, Stock S Patricia
Department of Entomology, University of Arizona, 1140 E. South Campus Dr., Tucson, AZ 85721-0036, USA.
Syst Parasitol. 2009 Feb;72(2):127-41. doi: 10.1007/s11230-008-9166-2. Epub 2008 Dec 30.
A new entomopathogenic nematode, Steinernema boemarei n. sp., is described from southern France. Morphological, molecular (28S and ITS rDNA sequence data) and cross-hybridisation studies were used for diagnostics and identification purposes. Both molecular and morphological data indicate that the new species belongs to the 'glaseri-group' of Steinernema spp. Key morphological diagnostic traits for S. boemarei n. sp. include the presence of prominent deirids (cervical papillae) on adult males, the morphology of the spicules and gubernaculum, and the arrangement of the 23 genital papillae of the first generation males. Additionally, morphometric traits of the third-stage infective juvenile, including total body length (mean 1,103 micron), tail length (mean 86 micron), location of the excretory pore (mean 91 micron), and D% (mean 63), E% (mean 106) and H% (mean 41) values are definitive. In addition to these morphological characters, analysis of both 28S and ITS rDNA sequences depict this Steinernema species as a distinct and unique entity.
一种新的昆虫病原线虫——博马雷斯氏斯氏线虫(Steinernema boemarei n. sp.)在法国南部被发现并予以描述。利用形态学、分子生物学(28S和ITS rDNA序列数据)以及交叉杂交研究进行诊断和鉴定。分子和形态学数据均表明该新物种属于斯氏线虫属的“格氏类群”。博马雷斯氏斯氏线虫(Steinernema boemarei n. sp.)的关键形态学诊断特征包括成年雄虫上显著的排泄孔(颈乳突)的存在、交合刺和引带的形态,以及第一代雄虫23个生殖乳突的排列。此外,第三期感染性幼虫的形态测量特征,包括虫体全长(平均1103微米)、尾长(平均86微米)、排泄孔位置(平均91微米)以及D%(平均63)、E%(平均106)和H%(平均41)值具有决定性意义。除了这些形态特征外,对28S和ITS rDNA序列的分析表明该斯氏线虫物种是一个独特的个体。