Nguyen Khuong B, Duncan Larry W
J Nematol. 2002 Jun;34(2):159-70.
A nematode collected from Diaprepes abbreviatus is identified and described as a new species, Steinernema diaprepesi n. sp. The new species is closely related to S. feltiae, S. glaseri, and S. oregonense and can be distinguished from these species by the following characteristics: Males: Spicule averaging 79 (71-90) microm and spicule shape; D% (distance from anterior end to excretory pore/ esophagus length x 100) about 80; the ratio SW (spicule length/anal body width) about 1.8. Females: Vulva with short, double- flapped epiptygma; tail terminus usually with 5 papillae-like structures. Infective juveniles: Body averaging 1,002 (880-1,133) microm, EP (distance from anterior end to excretory pore) = 74 (66-83) microm; tail length = 83 (65-91) microm, and E% (EP/tail length x 100) = 89.6 (78-114). Lateral field pattern variable, the formula for the arrangement of ridges from head to tail is: 2, 6, 7, 8, 4, 2. The portion with eight ridges is the longest. This new species can be differentiated further from three closest species (S. feltiae, S. glaseri, and S. oregonense) by characteristic sequences of their ITS regions, including sequence lengths, ratios of similarity, composition, and differences in base characters in sequence alignment.
从甘蔗金龟子(Diaprepes abbreviatus)体内采集到的一种线虫被鉴定并描述为一个新物种,即甘蔗斯氏线虫(Steinernema diaprepesi n. sp.)。该新物种与食菌斯氏线虫(S. feltiae)、格氏斯氏线虫(S. glaseri)和俄勒冈斯氏线虫(S. oregonense)密切相关,可通过以下特征与这些物种区分开来:雄虫:交合刺平均长度为79(71 - 90)微米及交合刺形状;D%(前端到排泄孔的距离/食管长度×100)约为80;SW(交合刺长度/肛门处体宽)比值约为1.8。雌虫:阴门有短的、双叶状的外阴盖;尾端通常有5个乳头状结构。感染性幼虫:虫体平均长度为1,002(880 - 1,133)微米,EP(前端到排泄孔的距离) = 74(66 - 83)微米;尾长 = 83(65 - 91)微米,E%(EP/尾长×100) = 89.6(78 - 114)。侧区模式可变,从头至尾的脊纹排列公式为:2, 6, 7, 8, 4, 2。有八条脊纹的部分最长。通过其ITS区域的特征序列,包括序列长度、相似性比值、组成以及序列比对中碱基特征的差异,该新物种可进一步与三个最相近的物种(食菌斯氏线虫、格氏斯氏线虫和俄勒冈斯氏线虫)区分开来。