Foster D O, Frydman M L, Usher J R
Can J Physiol Pharmacol. 1977 Feb;55(1):52-64. doi: 10.1139/y77-008.
Barbital-sedated, cold-acclimated (CA) or warm-acclimated (WA) rats were given different doses and combinations of noradrenaline, theophylline, and the adrenergic-blocking agents propranolol and phentolamine, to stimulate or inhibit calorigenesis in various ways. To see whether the effects of these drugs on calorigenesis could be ascribed to effects on the adenylate cyclase (EC 4.6.1.1) - cyclic AMP system, and to try to assess thereby the significance of this system in the regulation of nonshivering thermogenesis (NST), changes in the concentration of plasma cyclic AMP were measured as an index (Broadus, A.E., Hardman, J.G., Kaminsky, N. I., Ball, J. H., Sutherland, E.W., and Liddle, G. W.: 1971. Ann. N.Y. Acad. Sci. 185, 50-60) of changes in tissue levels of cyclic AMP. In CA rats, which have a severalfold greater capacity for NST than WA rats, calorigenic responses to noradrenaline, theophylline, noradrenaline plus theophylline, or phentolamine plus theophylline were as much as four times larger than in WA rats, However, the changes in level of plasma cyclic AMP produced by each of these and other treatments were virtually the same for both groups. It would appear, therefore, that the difference between WA and CA rats in ability to produce heat by NST is not a function of the amplitude of changes in tissue levels of cyclic AMP. Nevertheless, it was also observed, and was particularly striking in CA rats, that when a drug or combination of drugs had a stimulatory, inhibitory, or synergistic effect on calorigenesis, it had a similar effect with respect to elevation of plasma cyclic AMP. Altogether, the results indicate that adenylate cyclase and cyclic AMP are likely to be participants in the regulation of NST in the rat, but that they would be subservient in this regard to whatever factors are responsible for acclimation-related differences in capacity for NST.
给巴比妥镇静、冷适应（CA）或热适应（WA）的大鼠给予不同剂量及组合的去甲肾上腺素、茶碱以及肾上腺素能阻滞剂普萘洛尔和酚妥拉明，以通过各种方式刺激或抑制产热。为了探究这些药物对产热的影响是否可归因于对腺苷酸环化酶（EC 4.6.1.1）-环磷酸腺苷系统的作用，并借此评估该系统在非寒战产热（NST）调节中的意义，将血浆环磷酸腺苷浓度的变化作为组织中环磷酸腺苷水平变化的指标进行测量（布罗德斯，A.E.，哈德曼，J.G.，卡明斯基，N.I.，鲍尔，J.H.，萨瑟兰，E.W.，利德尔，G.W.：1971年。《纽约科学院学报》185卷，50 - 60页）。在NST能力比WA大鼠高几倍的CA大鼠中，对去甲肾上腺素、茶碱、去甲肾上腺素加茶碱或酚妥拉明加茶碱的产热反应比WA大鼠大达四倍。然而，两组中这些及其他处理所产生的血浆环磷酸腺苷水平变化实际上是相同的。因此，看来WA大鼠和CA大鼠通过NST产热能力的差异并非组织中环磷酸腺苷水平变化幅度的函数。尽管如此，还观察到，并且在CA大鼠中尤为显著的是，当一种药物或药物组合对产热有刺激、抑制或协同作用时，它对血浆环磷酸腺苷升高也有类似作用。总体而言，结果表明腺苷酸环化酶和环磷酸腺苷可能参与大鼠NST的调节，但在这方面它们服从于导致NST能力与适应相关差异的任何因素。
