Tajer Mohammad Ghazvini Parisa, Ghorbanzadeh Mashkani Saeid
Department of Nuclear Biotechnology, Nuclear Science Research School, Nuclear Science and Technology Research Institute, North Karegar Street, Tehran, Iran.
Bioresour Technol. 2009 Apr;100(8):2361-8. doi: 10.1016/j.biortech.2008.11.025. Epub 2008 Dec 30.
Thirty-eight soil samples were collected from crude oil contaminated land in south of Iran. Initial screening of a total of 100 bacterial isolates, resulted in the selection of one isolate with maximum adsorption capacity of 52.7 mg vanadate/g dry weight. It was tentatively identified as Halomonas sp. according to morphological and biochemical properties and named strain GT-83. Removal of vanadate by biosorption with Halomonas sp. GT-83 was very sensitive to solution pH. Vanadate adsorption decreased with increasing pH, with maximum adsorption capacities achieved in at pH 3.0 in the absence and in the presence of increasing concentrations of salt. Vanadate-salt biosorption studies were also performed at this pH value. Equilibrium uptakes of vanadate increased with increasing vanadate concentration up to 600 mg/l. Maximum metal removal (91.8%) took place at pH 3.0 with initial vanadate concentration of 100mg/l, which got reduced (84.8%) in the presence of 50 g/l salt. The equilibrium sorption data were analyzed by using Freundlich isotherm. The specific uptake of vanadate increased at low cell concentration and decreased when cell concentration exceeded 0.75 g/l. The paper also demonstrates the potential value of micro-PIXE in biosorption studies.
从伊朗南部原油污染土地采集了38个土壤样本。对总共100株细菌分离株进行初步筛选后，选出了一株吸附能力最强的分离株，其钒酸盐吸附量为52.7毫克/克干重。根据形态和生化特性，初步鉴定为嗜盐单胞菌属，并命名为GT-83菌株。嗜盐单胞菌属GT-83菌株对钒酸盐的生物吸附去除对溶液pH值非常敏感。钒酸盐吸附量随pH值升高而降低，在不存在盐和存在不同浓度盐的情况下，pH值为3.0时吸附量最大。还在此pH值下进行了钒酸盐-盐生物吸附研究。钒酸盐的平衡摄取量随钒酸盐浓度增加而增加，直至600毫克/升。在pH值为3.0、初始钒酸盐浓度为100毫克/升时，最大金属去除率为91.8%，在存在50克/升盐的情况下，该去除率降至84.8%。采用Freundlich等温线对平衡吸附数据进行分析。在低细胞浓度下，钒酸盐的比摄取量增加，而当细胞浓度超过0.75克/升时则降低。本文还展示了微束质子激发X射线发射分析在生物吸附研究中的潜在价值。
