Kawakita Hidetaka, Yoshimura Yuko, Hamamoto Kohshi, Seto Hirokazu, Ohto Keisuke, Harada Hiroyuki, Inoue Katsutoshi
Department of Applied Chemistry, Faculty of Science and Engineering, Saga University, Saga 840-8502, Japan.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Jan 15;877(3):347-50. doi: 10.1016/j.jchromb.2008.12.031. Epub 2008 Dec 24.
Dextransucrase forms a complex with dextran during an enzymatic reaction with sucrose. Using its enzymatic character, we performed a continuous and dynamic rejection of colloidal particles by generating dextran with dextransucrase immobilized in an inorganic porous membrane. Inorganic membranes having 1.9 and 3.0 U/g of immobilized dextransucrase, and 4.1 and 9.4 mg/g of generated dextran, respectively, had constant rejection percentages for 55 and 100 nm colloidal particles in permeating solutions. On the other hand, permeating sucrose solutions containing colloidal particles through a dextran-immobilized membrane dynamically increased the rejection percentages of the colloidal particles owing to dextran generation via enzymatic reaction. The dynamic increase was due to the gradually generating dextran dynamically occupying the membrane pore with its steric volume.
在与蔗糖的酶促反应过程中，葡聚糖蔗糖酶会与葡聚糖形成复合物。利用其酶促特性，我们通过将固定在无机多孔膜中的葡聚糖蔗糖酶生成葡聚糖，实现了对胶体颗粒的连续动态截留。固定有1.9和3.0 U/g葡聚糖蔗糖酶、分别生成4.1和9.4 mg/g葡聚糖的无机膜，对渗透溶液中55和100 nm的胶体颗粒具有恒定的截留率。另一方面，含有胶体颗粒的蔗糖溶液透过固定有葡聚糖的膜时，由于酶促反应生成葡聚糖，胶体颗粒的截留率会动态增加。这种动态增加是由于逐渐生成的葡聚糖以其空间体积动态占据膜孔所致。
