Wang Yu-Yan, Gao Jian-Hua, Jiang Ping, Lu Feng, Liao Yun-Jun
Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Zhonghua Zheng Xing Wai Ke Za Zhi. 2008 Sep;24(5):381-4.
To investigate the surface markers of adipose-derived stromal cells (ASCs) with high potential of adipogenic differentiation for increasing efficiency of adipose tissue engineering with selected seeds cells.
ASCs were harvested from human adipose tissue by collagenase digestion. After proliferation and adipogenic induction of ASCs, the mature induced adipocytes floating in the induction medium were collected. Ceiling culture was used to culture adipocytes and then dedifferentiated adipocytes was obtained at the ceiling. The reproductive activity, adipogenic differentiation potency and expression of surface markers were compared between the dedifferentiated adipocytes and ASCs.
The reproductive activity between the dedifferentiation adipocytes and ASCs were similar. The potential of adipogenic differentiation of the dedifferentiated adipocytes was stronger than that of the ASCs. The expression of cell surface markers of both cells were almost the same. But the CD54 positive expression in dedifferentiated adipocytes was higher than that in ASCs.
The CD54 expression maybe closely associated with high potential of adipogenic differentiation of dedifferentiated adipocytes. CU54 maybe the specific identification of cell surface marker of ASCs with high adipogenic differentiation.
研究具有高成脂分化潜能的脂肪来源基质细胞（ASCs）的表面标志物，以提高脂肪组织工程种子细胞的筛选效率。
采用胶原酶消化法从人脂肪组织中获取ASCs。对ASCs进行增殖和成脂诱导后，收集诱导培养基中漂浮的成熟诱导脂肪细胞。采用悬滴培养法培养脂肪细胞，然后在悬滴处获得去分化脂肪细胞。比较去分化脂肪细胞和ASCs的增殖活性、成脂分化潜能及表面标志物表达。
去分化脂肪细胞和ASCs的增殖活性相似。去分化脂肪细胞的成脂分化潜能强于ASCs。两种细胞的细胞表面标志物表达基本相同。但去分化脂肪细胞中CD54的阳性表达高于ASCs。
CD54表达可能与去分化脂肪细胞的高成脂分化潜能密切相关。CD54可能是高成脂分化ASCs细胞表面标志物的特异性识别分子。
