一项免疫荧光试验，用于确定患有直肠阴道狭窄的牛肌肉活检样本中II型胶原蛋白的存在情况。
An immunofluorescence test to determine the occurrence of type II collagen in muscle biopsies from cattle with rectovaginal constriction.
作者信息
Dawe D L, Lindl P A, Wallace C E, Brown J
机构信息
Department of Medical Microbiology, College of Veterinary Medicine, University of Georgia, Athens 30602.
出版信息
J Vet Diagn Invest. 1991 Jul;3(3):242-4. doi: 10.1177/104063879100300311.
Type II collagen occurs in the muscles of rectovaginal constriction (RVC) affected and carrier cattle but not in normal cattle. Muscle biopsies from known RVC affected and carrier cattle and normal cattle were examined for the presence of Type II collagen using affinity purified goat anti-collagen II serum in a fluorescent antibody test. Type II collagen was consistently found in RVC affected animals (22 of 23 samples score positive). Rectovaginal constriction carrier animals had variable staining for the Type II collagen (25 of 47 samples scored positive). Some positive staining was also observed in the control animals (8 of 34 samples scored positive). Because of the variable occurrence of Type II collagen, the value of fluorescent antibody staining to identify RVC carrier animals is uncertain.
II型胶原蛋白存在于受直肠阴道狭窄（RVC）影响的牛和携带该病症的牛的肌肉中，但正常牛的肌肉中不存在。使用亲和纯化的山羊抗II型胶原蛋白血清，通过荧光抗体试验对已知受RVC影响的牛、携带该病症的牛以及正常牛的肌肉活检样本进行检测，以确定是否存在II型胶原蛋白。在受RVC影响的动物中始终能检测到II型胶原蛋白（23个样本中有22个呈阳性）。直肠阴道狭窄携带动物的II型胶原蛋白染色情况不一（47个样本中有25个呈阳性）。在对照动物中也观察到一些阳性染色（34个样本中有8个呈阳性）。由于II型胶原蛋白的出现情况不一，荧光抗体染色用于识别RVC携带动物的价值尚不确定。
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