Lorente Carmen, Sainz Angel, Tesouro Miguel A
Veterinary Faculty, Universidad CEU Cardenal Herrera, Valencia and ADERVET, Madrid, Spain.
Ann N Y Acad Sci. 2008 Dec;1149:114-7. doi: 10.1196/annals.1428.040.
The purpose of this study was the characterization of the different subsets of lymphocyte cells in dogs with subclinical ehrlichiosis in order to contribute to the knowledge of the immune response developed in the course of this disease. Thirty-eight dogs with subclinical ehrlichiosis and 15 unaffected dogs from two shelters in the area of Valencia (eastern Spain) were included in the study. The study of lymphocyte populations was made by flow cytometry. Monoclonal antibodies against CD3, CD4, CD8, and CD21 were used. Based on our results, the most common findings of the subclinical phase of canine ehrlichiosis were lymphocytosis, relative neutropenia, and a decrease in the neutrophil/lymphocyte ratio. Lymphocytosis in these dogs was from an increase of T lymphocyte counts. Tc cell counts in dogs with subclinical ehrlichiosis were higher than in healthy dogs. This rise in the number of Tc lymphocytes resulted in a reduced percentage of Th lymphocytes and in a decrease in the CD4/CD8 ratio.
本研究的目的是对亚临床型埃立克体病犬的不同淋巴细胞亚群进行特征分析，以便增进对该病病程中所产生免疫反应的了解。本研究纳入了来自西班牙东部巴伦西亚地区两个收容所的38只亚临床型埃立克体病犬和15只未受影响的犬。通过流式细胞术对淋巴细胞群体进行研究。使用了针对CD3、CD4、CD8和CD21的单克隆抗体。根据我们的结果，犬埃立克体病亚临床阶段最常见的表现为淋巴细胞增多、相对中性粒细胞减少以及中性粒细胞/淋巴细胞比值降低。这些犬的淋巴细胞增多是由于T淋巴细胞计数增加。亚临床型埃立克体病犬的Tc细胞计数高于健康犬。Tc淋巴细胞数量的增加导致Th淋巴细胞百分比降低以及CD4/CD8比值下降。
