Yoneyama F, Fukao M, Zendo T, Nakayama J, Sonomoto K
Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, Higashi-ku, Fukuoka, Japan.
J Appl Microbiol. 2008 Dec;105(6):1982-90. doi: 10.1111/j.1365-2672.2008.03958.x.
To characterize the genetic and biochemical features of nisin Q.
The nisin Q gene cluster was sequenced, and 11 putative orfs having 82% homology with the nisin A biosynthesis gene cluster were identified. Nisin Q production was confirmed from the nisQ-introduced nisin Z producer. In the reporter assay, nisin Q exhibited an induction level that was threefold lower than that of nisin A. Nisin Q demonstrated an antimicrobial spectrum similar to those of the other nisins. Under oxidizing conditions, nisin Q retained a higher level of activity than nisin A. This higher oxidative tolerance could be attributed to the presence of only one methionine residue in nisin Q, in contrast to other nisins that contain two.
The 11 orfs of the nisin producers were identical with regard to their functions. The antimicrobial spectra of the three natural nisins were similar. Nisin Q demonstrated higher oxidative tolerance than nisin A.
Genetic and biochemical features of nisin Q are similar to those of other variants. Moreover, owing to its higher oxidative tolerance, nisin Q is a potential alternative for nisin A.
表征乳酸链球菌素Q的遗传和生化特性。
对乳酸链球菌素Q基因簇进行测序，鉴定出11个与乳酸链球菌素A生物合成基因簇具有82%同源性的推定开放阅读框。从导入nisQ的乳酸链球菌素Z产生菌中证实了乳酸链球菌素Q的产生。在报告基因检测中，乳酸链球菌素Q的诱导水平比乳酸链球菌素A低三倍。乳酸链球菌素Q的抗菌谱与其他乳酸链球菌素相似。在氧化条件下，乳酸链球菌素Q比乳酸链球菌素A保留更高水平的活性。这种更高的氧化耐受性可归因于乳酸链球菌素Q中仅存在一个甲硫氨酸残基，而其他乳酸链球菌素含有两个。
乳酸链球菌素产生菌的11个开放阅读框在功能上是相同的。三种天然乳酸链球菌素的抗菌谱相似。乳酸链球菌素Q比乳酸链球菌素A表现出更高的氧化耐受性。
乳酸链球菌素Q的遗传和生化特性与其他变体相似。此外，由于其更高的氧化耐受性，乳酸链球菌素Q是乳酸链球菌素A的潜在替代品。
