Kogan E M, Dyatlova N G, Sidorova N S, Vilner L M, Vrána O, Brabec V
Institute of Macromolecular Compounds, Academy of Sciences of the USSR, Leningrad.
Biophys Chem. 1991 Jul;40(3):231-9. doi: 10.1016/0301-4622(91)80023-k.
Conformation of double-stranded complexes of polyriboguanylic acid with halogenated polyribocytidylic acid [poly(C)] was studied with the aid of differential pulse polarography, terbium fluorescence and circular dichroism spectrometry. It was shown that halogenation at C(5) of cytosine residues in poly(C) disturbed the ordered structure of the double-helical complex. In addition, this halogenation does not improve antiviral activity of the polynucleotide complex studied in the system of vesicular stomatitis virus and the cell culture of chicken embryos. It was concluded that the regularity of the secondary structure of synthetic RNAs might play an important role in the mechanism of biological activity of these biomacromolecules.
借助差分脉冲极谱法、铽荧光法和圆二色光谱法研究了聚核糖鸟苷酸与卤化聚核糖胞苷酸[聚（C）]双链复合物的构象。结果表明，聚（C）中胞嘧啶残基的C（5）位卤化扰乱了双螺旋复合物的有序结构。此外，这种卤化并不能提高在水泡性口炎病毒和鸡胚细胞培养系统中所研究的多核苷酸复合物的抗病毒活性。得出的结论是，合成RNA二级结构的规则性可能在这些生物大分子的生物活性机制中起重要作用。
