Sàbat Maria, Lo Nostro Fabiana, Casadevall Margarida, Muñoz Marta
Area de Biologia Animal, Dpt. de Ciències Ambientals, Universitat de Girona, Girona, Spain.
J Morphol. 2009 Jun;270(6):662-72. doi: 10.1002/jmor.10707.
The testicular organization and semicystic spermatogenesis of Scorpaena porcus and Scorpaena scrofa are analyzed by means of optic and electron microscopy and immunohistochemical techniques. The testicular structure of S. porcus and S. scrofa belongs to the unrestricted spermatogonial type, but has typical features of the restricted type. Moreover, the structure presents an epithelioid arrangement of Sertoli and germ cells rather than the germinal epithelium that appears in the majority of teleosts. After the cysts open, Sertoli cells hypertrophy and remain on the basement membrane, linked by interdigitations and tight junctions and bordering the lumen of the lobule, which at this moment works as an efferent duct. Secretions of Sertoli cells usually function in the nutrition of germ cells, and they seem to contribute in it even in this kind of spermatogenesis in which the free lumen spermatids do not have any connection with Sertoli cells. In addition, Sertoli cells can divide after the cysts have broken apart and hypertrophied, suggesting that they are still important for the final maturation of spermatozoa and seminal fluid formation.
通过光学显微镜、电子显微镜和免疫组织化学技术，对黑鲉和欧洲鲉的睾丸组织及半囊状精子发生进行了分析。黑鲉和欧洲鲉的睾丸结构属于无限制精原细胞型，但具有限制型的典型特征。此外，该结构呈现出支持细胞和生殖细胞的上皮样排列，而非大多数硬骨鱼中出现的生殖上皮。囊肿开放后，支持细胞肥大并留在基底膜上，通过指状交叉和紧密连接相连，并与小叶腔相邻，此时小叶腔起到输出小管的作用。支持细胞的分泌物通常为生殖细胞提供营养，即使在这种游离腔精子细胞与支持细胞没有任何联系的精子发生过程中，它们似乎也发挥着作用。此外，囊肿破裂并肥大后，支持细胞能够分裂，这表明它们对精子的最终成熟和精液形成仍然很重要。
