Guo Dan, Xiong Yang, Zhang Yue, Wu Zhongbin, Cui Lili, Chen Jianming
Department of Pharmaceutical Science, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Jan 15;877(3):323-7. doi: 10.1016/j.jchromb.2008.12.007. Epub 2008 Dec 9.
A LC/MS/MS method was developed and validated for determination of nobiliside A in rat plasma. Analyses were separated by an Agella Venusil ASB-C18 column and isocratic elution with methanol:water (80:20, v/v) as a mobile phase. A tandem mass spectrometer was used as a detector for quantitative analysis. Calibration curves (R(2)>0.999) in spiked plasma were linear over the concentration range of 50-5000 ng/mL. The overall accuracy of this method was 93-104% for nobiliside A. Within-day and between-day variability was both less than 9% in plasma. The data indicate that the LC/MS/MS method is an effective method for the pharmacokinetics study of nobiliside A in rat plasma.
建立并验证了一种用于测定大鼠血浆中诺比利苷A的液相色谱-串联质谱（LC/MS/MS）方法。采用Agella Venusil ASB-C18色谱柱进行分析，以甲醇:水（80:20，v/v）作为流动相进行等度洗脱。使用串联质谱仪作为检测器进行定量分析。加标血浆中的校准曲线（R(2)>0.999）在50-5000 ng/mL的浓度范围内呈线性。该方法对诺比利苷A的总体准确度为93-104%。血浆中的日内和日间变异均小于9%。数据表明，LC/MS/MS方法是研究诺比利苷A在大鼠血浆中药物动力学的有效方法。
