一种用于定量大鼠血浆中诺比利甙A的液相色谱/串联质谱法的开发与验证
Development and validation of a LC/MS/MS method for quantification of nobiliside A in rat plasma.
作者信息
Guo Dan, Xiong Yang, Zhang Yue, Wu Zhongbin, Cui Lili, Chen Jianming
机构信息
Department of Pharmaceutical Science, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Jan 15;877(3):323-7. doi: 10.1016/j.jchromb.2008.12.007. Epub 2008 Dec 9.
A LC/MS/MS method was developed and validated for determination of nobiliside A in rat plasma. Analyses were separated by an Agella Venusil ASB-C18 column and isocratic elution with methanol:water (80:20, v/v) as a mobile phase. A tandem mass spectrometer was used as a detector for quantitative analysis. Calibration curves (R(2)>0.999) in spiked plasma were linear over the concentration range of 50-5000 ng/mL. The overall accuracy of this method was 93-104% for nobiliside A. Within-day and between-day variability was both less than 9% in plasma. The data indicate that the LC/MS/MS method is an effective method for the pharmacokinetics study of nobiliside A in rat plasma.
建立并验证了一种用于测定大鼠血浆中诺比利苷A的液相色谱-串联质谱（LC/MS/MS）方法。采用Agella Venusil ASB-C18色谱柱进行分析，以甲醇:水（80:20，v/v）作为流动相进行等度洗脱。使用串联质谱仪作为检测器进行定量分析。加标血浆中的校准曲线（R(2)>0.999）在50-5000 ng/mL的浓度范围内呈线性。该方法对诺比利苷A的总体准确度为93-104%。血浆中的日内和日间变异均小于9%。数据表明，LC/MS/MS方法是研究诺比利苷A在大鼠血浆中药物动力学的有效方法。
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