Yoshida Makoto, Sato Kan, Kaneko Satoshi, Fukuda Kiyoharu
Department of Environmental and Natural Resource Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology.
Biosci Biotechnol Biochem. 2009 Jan;73(1):67-73. doi: 10.1271/bbb.80477. Epub 2009 Jan 7.
We searched the genome database of the basidiomycete Coprinopsis cinerea (Coprinus cinereus) and found five genes encoding the glycoside hydrolase family 6 (GH6) enzyme, CcCel6A, CcCel6B, CcCel6C, CcCel6D, and CcCel6E, designated in order of increasing locus number (CC1G_01107.1, CC1G_04166.1, CC1G_08276.1, CC1G_08277.1, and CC1G_10605.1). The amino acid sequence of CcCel6A suggests a two-domain structure consisting of an N-terminal family 1 carbohydrate-binding module (CBM1) and a GH6 catalytic domain, while the other genes lack CBM1. The transcripts of CcCel6A were observed at the active growth stage in cellulose culture, whereas they were absent from glucose culture. Cellobiose strongly induced transcription of CcCel6A. On the other hand, transcripts of CcCel6B, -D, and -E were detected in both glucose and cellulose cultures, and transcription of them was induced weakly by cellobiose. The transcript level of CcCel6C was not influenced by glucose or cellobiose.
我们搜索了担子菌灰盖鬼伞（Coprinus cinereus）的基因组数据库，发现了五个编码糖苷水解酶家族6（GH6）酶的基因，即CcCel6A、CcCel6B、CcCel6C、CcCel6D和CcCel6E，按照基因座编号递增顺序命名（CC1G_01107.1、CC1G_04166.1、CC1G_08276.1、CC1G_08277.1和CC1G_10605.1）。CcCel6A的氨基酸序列显示其具有由N端家族1碳水化合物结合模块（CBM1）和GH6催化结构域组成的双结构域结构，而其他基因缺乏CBM1。在纤维素培养的活跃生长阶段观察到CcCel6A的转录本，而在葡萄糖培养中则未检测到。纤维二糖强烈诱导CcCel6A的转录。另一方面，在葡萄糖和纤维素培养中均检测到CcCel6B、-D和-E的转录本，纤维二糖对它们的转录有微弱诱导作用。CcCel6C的转录水平不受葡萄糖或纤维二糖的影响。
