Ohya Susumu, Kimura Kazunori, Niwa Satomi, Ohno Akitoshi, Kojima Yoshiyuki, Sasaki Shoichi, Kohri Kenjiro, Imaizumi Yuji
Department of Molecular and Cellular Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan.
J Pharmacol Sci. 2009 Jan;109(1):148-51. doi: 10.1254/jphs.08208sc. Epub 2009 Jan 8.
K(+) channels are key molecules in the progression of several cancer types and considered to be potential targets for cancer therapy. We examined the gene expressions of voltage-gated (K(v)), Ca(2+)-activated (K(Ca)), and two-pore domain (K(2P)) K(+)-channel subtypes in needle-biopsy samples of human prostate cancer (PCa) by real-time PCR and compared them with those in PCa epithelial cell lines. The expression of K(v)1.3, K(Ca)1.1, K(Ca)3.1, and K(2P)1 markedly increased in the PCa group with Gleason score of 5 - 6 (GS5-6) but significantly decreased in the GS8-9 group. This malignancy grade-dependent K(+)-channel expression pattern may provide a convenient marker to understand PCa progression level.
钾离子通道是几种癌症类型进展过程中的关键分子，被认为是癌症治疗的潜在靶点。我们通过实时聚合酶链反应检测了人类前列腺癌（PCa）针吸活检样本中电压门控性（K(v)）、钙激活（K(Ca)）和双孔结构域（K(2P)）钾离子通道亚型的基因表达，并将其与PCa上皮细胞系中的表达进行比较。在Gleason评分为5 - 6（GS5-6）的PCa组中，K(v)1.3、K(Ca)1.1、K(Ca)3.1和K(2P)1的表达显著增加，但在GS8-9组中显著降低。这种恶性程度依赖性的钾离子通道表达模式可能为了解PCa进展水平提供一个便捷的标志物。
