致病性弧菌中TonB2簇TtpC蛋白的分子与遗传特征分析
Molecular and genetic characterization of the TonB2-cluster TtpC protein in pathogenic vibrios.
作者信息
Kuehl Carole J, Crosa Jorge H
机构信息
Department of Molecular Microbiology and Immunology, Oregon Health and Science University, Portland, OR 97239, USA.
出版信息
Biometals. 2009 Feb;22(1):109-15. doi: 10.1007/s10534-008-9194-z. Epub 2009 Jan 8.
Abstract
TtpC is a fourth required protein in the TonB2 energy transduction system in Vibrio anguillarum. TtpC is necessary for iron transport mediated by the TonB2 system and is highly conserved in all pathogenic vibrio species studied to date as well as several marine organisms. We show here that the TtpC proteins from selected pathogenic vibrio species can function with the TonB2 system of V. anguillarum to allow iron transport mediated by a chimeric TonB2 system where the native ExbB2, ExbD2 and TonB2 function with an episomally expressed TtpC in trans from a different species. The discovery that inter-species complementation occurs can be used to identify the functional regions of the TtpC proteins and will lead to an investigation of the mechanism of interaction between the TtpC protein and other members of the TonB2 system.
摘要
TtpC是鳗弧菌中TonB2能量转导系统所需的第四种蛋白质。TtpC对于由TonB2系统介导的铁转运是必需的，并且在迄今为止研究的所有致病性弧菌物种以及几种海洋生物中高度保守。我们在此表明，来自选定致病性弧菌物种的TtpC蛋白可以与鳗弧菌的TonB2系统一起发挥作用，从而允许由嵌合TonB2系统介导铁转运，其中天然的ExbB2、ExbD2和TonB2与来自不同物种的游离表达的TtpC在反式中发挥作用。种间互补发生这一发现可用于鉴定TtpC蛋白的功能区域，并将导致对TtpC蛋白与TonB2系统其他成员之间相互作用机制的研究。
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