Herlitz H, Hamberger L, Ahrén K
Horm Metab Res. 1977 Jan;9(1):81-5. doi: 10.1055/s-0028-1093589.
Prepubertal rat ovaries were incubated in medium containing the non-utilizable amino acids alpha-aminoisobutyric acid (AIB-14C) or 1-aminocyclo-pentane-carboxylic acid (cycloleucine-14C). The rate of uptake of the two amino acids was studied in the isolated ovaries after different incubation periods. Addition of 5mM cyclic AMP (cAMP) caused a slight stimulation of the AIB-transport but in higher concentrations (10-25 mM) an inhibition was noted. With dibutyrl cyclic AMP (dbcAMP) a dose-dependent increase was seen with 0.5-5 mM concentrations with no further effect of higher concentrations. Time course studies were performed with both AIB and cycloleucine in presence of 10 mM dbcAMP and increased uptake values were noted at each time studied (30-240 min). The phosphodiesterase inhibitor aminophyline in lower concentrations did not influence AIB-transport but 5-10 mM caused increased uptake values in the ovaries. The stimulatory action of dbcAMP on amino acid transport was augmented by a low concentration of aminophylline (0.5 mM). Experiments were in addition carried out in the presence of puromycin and under these circumstances it was still possible to enhance amino acid transport by addition of dbcAMP. The results are discussed in relation to earlier reported effects of gonadotropins on ovarian amino acid transport.
将青春期前大鼠的卵巢置于含有不可利用氨基酸α-氨基异丁酸（AIB-14C）或1-氨基环戊烷羧酸（环亮氨酸-14C）的培养基中培养。在不同孵育时间后，研究了分离卵巢中这两种氨基酸的摄取速率。添加5mM环磷酸腺苷（cAMP）对AIB转运有轻微刺激作用，但在较高浓度（10 - 25mM）时则出现抑制作用。对于二丁酰环磷酸腺苷（dbcAMP），在0.5 - 5mM浓度下可见剂量依赖性增加，更高浓度则无进一步影响。在10mM dbcAMP存在的情况下，对AIB和环亮氨酸进行了时间进程研究，在每个研究时间点（30 - 240分钟）均记录到摄取值增加。较低浓度的磷酸二酯酶抑制剂氨茶碱不影响AIB转运，但5 - 10mM会使卵巢中的摄取值增加。低浓度氨茶碱（0.5mM）增强了dbcAMP对氨基酸转运的刺激作用。此外，在嘌呤霉素存在的情况下进行了实验，在这种情况下，添加dbcAMP仍有可能增强氨基酸转运。本文结合先前报道的促性腺激素对卵巢氨基酸转运的影响对结果进行了讨论。
