用等温滴定量热法监测 RNA 的多步折叠途径。
Monitoring of an RNA multistep folding pathway by isothermal titration calorimetry.
机构信息
RNA Group, Département de Biochimie, Université de Sherbrooke, Sherbrooke, Québec, Canada.
出版信息
Biophys J. 2009 Jan;96(1):132-40. doi: 10.1016/j.bpj.2008.09.033.
Abstract
Isothermal titration calorimetry was used to monitor the energetic landscape of a catalytic RNA, specifically that of the hepatitis delta virus ribozyme. Using mutants that isolated various tertiary interactions, the thermodynamic parameters of several ribozyme-substrate intermediates were determined. The results shed light on the impact of several tertiary interactions on the global structure of the ribozyme. In addition, the data indicate that the formation of the P1.1 pseudoknot is the limiting step of the molecular mechanism. Last, as illustrated here, isothermal titration calorimetry appears to be a method of choice for the elucidation of an RNA's folding pathway.
摘要
等温滴定量热法被用于监测催化 RNA 的能量景观，特别是乙型肝炎 delta 病毒核酶。通过使用分离各种三级相互作用的突变体，确定了几种核酶-底物中间物的热力学参数。结果揭示了几种三级相互作用对核酶整体结构的影响。此外，数据表明 P1.1 假结的形成是分子机制的限速步骤。最后，如这里所示，等温滴定量热法似乎是阐明 RNA 折叠途径的首选方法。
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