Zhou Xin-Li, He Hui, Liu Bao-Lin, Hua Tse-Chao, Chen Ying
Institute of Biothermal Sciences, University of Shanghai for Science and Technology, Shanghai 200093, China.
Cryo Letters. 2008 Jul-Aug;29(4):285-92.
Successful storage of red blood cells (RBCs) by freeze-drying technique has important implications in blood transfusion and clinical medicine. We presented a method of preservation RBCs by pretreating them with glycerol solution and then freeze-drying. The effects of glycerol pretreatment on recovery and antioxidant enzyme activities of lyophilized RBCs were investigated. Rehydration of pretreated in 40 percent glycerol and freeze-dried RBCs resulted in 55.3+/-4.3 percent numerical recovery and 53.5+/-3.9 percent hemoglobin recovery, which were significantly higher than freeze-dried RBCs without glycerol pretreatment (P less than 0.01). Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-PX) activities of 40 percent glycerol pretreated and freeze-dried RBCs almost remained fully active after freeze-drying. After 180 days storage, the activities decreased by 25.6 percent , 21.5 percent and 18.4 percent, respectively, which are significantly less than those of freeze-dried RBCs without glycerol pretreatment (P < 0.1). These data demonstrated that glycerol pretreatment had beneficial effects on the recovery and antioxidant enzyme activities of lyophilized RBCs.
通过冷冻干燥技术成功储存红细胞（RBCs）在输血和临床医学中具有重要意义。我们提出了一种通过用甘油溶液预处理红细胞然后进行冷冻干燥来保存红细胞的方法。研究了甘油预处理对冻干红细胞回收率和抗氧化酶活性的影响。用40%甘油预处理并冻干的红细胞复水后，数值回收率为55.3±4.3%，血红蛋白回收率为53.5±3.9%，显著高于未进行甘油预处理的冻干红细胞（P小于0.01）。40%甘油预处理并冻干的红细胞的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-PX）活性在冻干后几乎保持完全活性。储存180天后，活性分别下降了25.6%、21.5%和18.4%，显著低于未进行甘油预处理的冻干红细胞（P<0.1）。这些数据表明，甘油预处理对冻干红细胞的回收率和抗氧化酶活性具有有益影响。
