Dye D E, Karlen S, Rohrbach B, Staub O, Braathen L R, Eidne K A, Coombe D R
Curtin University of Technology, Molecular Immunology Group, Perth, Australia.
Cell Mol Life Sci. 2009 Feb;66(4):681-96. doi: 10.1007/s00018-009-8645-1.
hShroom1 (hShrm1) is a member of the Apx/Shroom (Shrm) protein family and was identified from a yeast two-hybrid screen as a protein that interacts with the cytoplasmic domain of melanoma cell adhesion molecule (MCAM). The characteristic signature of the Shrm family is the presence of a unique domain, ASD2 (Apx/Shroom domain 2). mRNA analysis suggests that hShrm1 is expressed in brain, heart, skeletal muscle, colon, small intestine, kidney, placenta and lung tissue, as well a variety of melanoma and other cell lines. Co-immunoprecipitation and bioluminescence resonance energy transfer (BRET) experiments indicate that hShrm1 and MCAM interact in vivo and by immunofluorescence microscopy some co-localization of these proteins is observed. hShrm1 partly co-localises with beta-actin and is found in the Triton X-100 insoluble fraction of melanoma cell extracts. We propose that hShrm1 is involved in linking MCAM to the cytoskeleton.
人源Shroom1（hShroom1）是Apx/Shroom（Shrm）蛋白家族的成员，通过酵母双杂交筛选被鉴定为一种与黑色素瘤细胞粘附分子（MCAM）胞质结构域相互作用的蛋白。Shrm家族的特征性标志是存在一个独特的结构域，即ASD2（Apx/Shroom结构域2）。mRNA分析表明，hShrm1在脑、心脏、骨骼肌、结肠、小肠、肾脏、胎盘和肺组织以及多种黑色素瘤和其他细胞系中表达。免疫共沉淀和生物发光共振能量转移（BRET）实验表明，hShrm1和MCAM在体内相互作用，通过免疫荧光显微镜观察到这些蛋白有一些共定位。hShrm1部分与β-肌动蛋白共定位，并且存在于黑色素瘤细胞提取物的Triton X-100不溶部分中。我们推测hShrm1参与将MCAM与细胞骨架连接起来。
