Mochalov S V, Kalenchuk V U, Gaĭnullina D K, Vorotnikov A V, Tarasova O S
Biofizika. 2008 Nov-Dec;53(6):1102-8.
The age-related dynamics of the activity of signalling pathways coupled to alpha1-adrenergic receptors and their dependence on the sympathetic innervation of arterial smooth muscle have been studied. The effects of the protein kinase C inhibitor (GF109203X, 10(-6) M) and the Rho-kinase inhibitor (Y27632, 10(-5) M) on the isometric contraction of the rat saphenous artery, induced by the alpha1-adrenoceptor agonist methoxamine, were examined. It was shown that the sensitivity to methoxamine of arteries from 2-week-old rats that are partially innervated was reduced as compared to adults, but the effects of both inhibitors were more prominent. The denervation induced by the excision of sympathetic ganglia increased the arterial sensitivity to methoxamine but was not accompanied by changes in sensitivity to the inhibitors. Therefore, the postnatal development of the arterial smooth muscle is characterized by a decrease in the contribution of protein kinase C and Rho-kinase to the regulation of contraction; however, these changes do not correlate with changes in the sensitivity of arteries to methoxamine and development of sympathetic innervation.
研究了与α1 - 肾上腺素能受体偶联的信号通路活性的年龄相关动态变化及其对动脉平滑肌交感神经支配的依赖性。研究了蛋白激酶C抑制剂（GF109203X，10^(-6) M）和Rho激酶抑制剂（Y27632，10^(-5) M）对α1 - 肾上腺素能受体激动剂甲氧明诱导的大鼠隐动脉等长收缩的影响。结果表明，与成年大鼠相比，2周龄部分神经支配的大鼠动脉对甲氧明的敏感性降低，但两种抑制剂的作用更显著。切除交感神经节引起的去神经支配增加了动脉对甲氧明的敏感性，但对抑制剂的敏感性没有变化。因此，动脉平滑肌的出生后发育特征是蛋白激酶C和Rho激酶对收缩调节的贡献降低；然而，这些变化与动脉对甲氧明的敏感性变化和交感神经支配的发育无关。
