Moticka E J, Adamus G
Department of Medical Microbiology and Immunology, Southern Illinois University School of Medicine, Springfield 62794-9230.
Cell Immunol. 1991 Nov;138(1):175-84. doi: 10.1016/0008-8749(91)90142-x.
Rhodopsin, an integral membrane protein of rod photoreceptor cells, induces an experimental autoimmune uveitis (EAU) when injected into Lewis rats. This disease is characterized by a mononuclear and polymorphonuclear cellular infiltrate of the retina resulting in destruction of the photoreceptor cells. In this study the B and T cell specificities of the response to bovine rhodopsin by Lewis rats were determined. Antibodies induced by injection of rhodopsin were directed almost exclusively to the IV-V loop (residues 174-202). Later in the response, antibody to the N-terminus was also detected. At the T cell level, most activity was directed to the C-terminus as measured by in vitro lymphocyte proliferation. Other minor T cell epitopes were found in the II-III (96-114) and IV-V (174-202) loops. Further dissection of the amino acid sequence responsible for the activity isolated to the C-terminus indicated that a 12-amino acid-long sequence (331-342) elicited the strongest proliferative response.
视紫红质是视杆光感受器细胞的一种整合膜蛋白，将其注射到Lewis大鼠体内会诱发实验性自身免疫性葡萄膜炎（EAU）。这种疾病的特征是视网膜出现单核和多形核细胞浸润，导致光感受器细胞遭到破坏。在本研究中，确定了Lewis大鼠对牛视紫红质反应的B细胞和T细胞特异性。注射视紫红质诱导产生的抗体几乎完全针对IV-V环（第174-202位氨基酸残基）。在反应后期，还检测到了针对N端的抗体。在T细胞水平，通过体外淋巴细胞增殖测定，大部分活性针对C端。在II-III环（第96-114位氨基酸残基）和IV-V环（第174-202位氨基酸残基）中发现了其他次要的T细胞表位。对负责C端活性的氨基酸序列进行进一步剖析表明，一个12个氨基酸长的序列（第331-342位氨基酸残基）引发了最强的增殖反应。
