间变性肾母细胞瘤表达转酮醇酶样酶1。
Anaplastic nephroblastomas express transketolase-like enzyme 1.
作者信息
Wu H-T, Allie N, Myer L, Govender D
机构信息
Division of Anatomical Pathology, National Health Laboratory Services, Groote-Schuur Hospital /Red Cross War Memorial Children's Hospital, University of Cape Town, Cape Town, South Africa.
出版信息
J Clin Pathol. 2009 May;62(5):460-3. doi: 10.1136/jcp.2008.063966. Epub 2009 Jan 12.
AIM
Transketolase-like enzyme 1 (TKTL1) is a glycolytic enzyme that has been found to be upregulated in several tumours, and it is associated with tumour progression. Nephroblastoma is the commonest paediatric renal malignancy and has a good prognosis except for those with anaplasia. To the best of the authors' knowledge, the expression of TKTL1 in nephroblastomas has not been studied before and the aim of this study was to compare the immunoexpression of TKTL1 in anaplastic and non-anaplastic nephroblastomas.
METHODS
Twenty-eight patients who had nephrectomies for nephroblastomas were studied. Archival formalin-fixed paraffin-wax-embedded tissue sections were stained with monoclonal TKTL1 antibody.
RESULTS
Six of the 15 anaplastic nephroblastomas showed staining in 80-100% of the tumour (p = 0.36). None of the 13 non-anaplastic nephroblastomas showed TKTL1 staining in >80% of the tumour.
CONCLUSION
TKTL1 expression is associated with the presence of anaplasia and may be a mechanism via which anaplastic tumour cells thrive under different conditions. Glycolytic inhibitors may play a role in anaplastic nephroblastomas.
目的
转酮醇酶样酶1（TKTL1）是一种糖酵解酶，已发现在多种肿瘤中上调，且与肿瘤进展相关。肾母细胞瘤是最常见的小儿肾脏恶性肿瘤，除间变者外预后良好。据作者所知，此前尚未研究过TKTL1在肾母细胞瘤中的表达，本研究的目的是比较TKTL1在间变性和非间变性肾母细胞瘤中的免疫表达。
方法
对28例行肾母细胞瘤肾切除术的患者进行研究。用单克隆TKTL1抗体对存档的福尔马林固定石蜡包埋组织切片进行染色。
结果
15例间变性肾母细胞瘤中有6例在80%-100%的肿瘤中呈染色阳性（p = 0.36）。13例非间变性肾母细胞瘤中无一例在超过80%的肿瘤中显示TKTL1染色阳性。
结论
TKTL1表达与间变的存在相关，可能是间变性肿瘤细胞在不同条件下得以生长的一种机制。糖酵解抑制剂可能在间变性肾母细胞瘤中发挥作用。
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