Kahnt W D, Mundy V, Grosch W
Z Lebensm Unters Forsch. 1975 Jun 30;158(2):77-82. doi: 10.1007/BF01460024.
2,6-dichlorophenol-indophenol is more stable in 75% ethanol than in aqueous solution and ascorbic acid reacts considerably faster in this media than glutathione. The activity determination of glutathione: dehydroascorbic acid oxidoreductase was based on these observations by a photometric measurement of the ascorbic acid resulting from the catalysis. Extracts from various types of wheat were tested and relatively high enzyme activities were found.
2,6 - 二氯酚靛酚在75%乙醇中比在水溶液中更稳定,且抗坏血酸在这种介质中与谷胱甘肽相比反应速度要快得多。谷胱甘肽:脱氢抗坏血酸氧化还原酶的活性测定基于这些观察结果,通过光度测量催化产生的抗坏血酸来进行。对各种类型小麦的提取物进行了测试,发现酶活性相对较高。