DNA中G×A错配的热力学：连续静电模型

Thermodynamics of G x A mispairs in DNA: continuum electrostatic model.

作者信息

Berashevich Julia, Chakraborty Tapash

机构信息

Department of Physics and Astronomy, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada.

出版信息

J Chem Phys. 2009 Jan 7;130(1):015101. doi: 10.1063/1.3050107.

DOI:10.1063/1.3050107

PMID:19140634

Abstract

An analysis of the stability of a duplex containing G x A mispairs or G x A/A x G tandem during the DNA melting has shown that the duplex stability depends on both DNA sequences and the conformations of the G x A mispairs. The thermodynamics of single pair opening for the G(anti) x A(syn) and G(anti) x A(anti) conformations adopted by a G x A mispair is found to strongly correlate with that of the canonical base pairs, while for the sheared conformation a significant difference is observed.

摘要

对包含G×A错配或G×A/A×G串联的双链体在DNA解链过程中的稳定性分析表明，双链体稳定性取决于DNA序列和G×A错配的构象。发现G×A错配所采用的G（反式）×A（顺式）和G（反式）×A（反式）构象的单碱基对打开的热力学与标准碱基对的热力学密切相关，而对于剪切构象则观察到显著差异。