Sibug R M, Stumpf W E, Shughrue P J, Hochberg R B, Drews U
Institute of Anatomy, University of Tübingen, F.R.G.
Brain Res Dev Brain Res. 1991 Jul 16;61(1):11-22. doi: 10.1016/0165-3806(91)90109-v.
The present study provides a detailed anatomical description of estrogen target cells in the mouse forebrain and pituitary gland during the sexual imprinting stage of the brain. Six 2-day-old mice (3 males and 3 females) were s.c. injected with 16 alpha-[125I]iodo-11 beta-methoxy-17 beta-estradiol ([125I]MIE2) and two additional mice (one male and one female) were s.c. injected with 1000x unlabeled 17 beta-estradiol 1 h before [125I]MIE2 to check the specificity of estradiol binding. Two hours after injection the mice were decapitated, the brains dissected, frozen sectioned, and processed for thaw mount autoradiography. The highest intensity of nuclear labeling was observed in the preoptic-anterior hypothalamic area, amygdala and cortex entorhinalis. Strong labeling was present in the cerebral cortex and moderate to strong labeling in the lateral septum, bed nucleus of stria terminalis and pituitary gland. Weak to moderate labeling was observed in the bulbus olfactorius, circumventricular organs, basal ganglia, ventral striatum, thalamus, hippocampus and pineal gland. No sex differences were observed in the intensity of labeling and distribution of the estrogen target sites. The topographic distributions of estrogen-concentrating cells in the hypothalamus of the 2-day-old mouse forebrain was similar to the adult pattern but differed prominently in the cerebral cortex, entorhinal cortex and thalamus: the cerebral cortex showed an extensive and intensive labeling, the intensity of labeling in the entorhinal cortex greatly exceeded that observed in the adult and the nucleus anterior medialis thalami was distinctly labeled.
本研究对大脑性印记阶段小鼠前脑和垂体中的雌激素靶细胞进行了详细的解剖学描述。6只2日龄小鼠（3只雄性和3只雌性）皮下注射16α-[¹²⁵I]碘-11β-甲氧基-17β-雌二醇（[¹²⁵I]MIE2），另外2只小鼠（1只雄性和1只雌性）在注射[¹²⁵I]MIE2前1小时皮下注射1000倍未标记的17β-雌二醇，以检查雌二醇结合的特异性。注射后2小时，将小鼠断头，解剖大脑，冷冻切片，并进行解冻贴片放射自显影处理。在视前-下丘脑前部区域、杏仁核和内嗅皮质观察到最高强度的核标记。大脑皮质有强标记，外侧隔、终纹床核和垂体有中度至强标记。在嗅球、室周器官、基底神经节、腹侧纹状体、丘脑、海马和松果体观察到弱至中度标记。在雌激素靶位点的标记强度和分布上未观察到性别差异。2日龄小鼠前脑下丘脑雌激素浓缩细胞的地形分布与成年模式相似，但在大脑皮质、内嗅皮质和丘脑有显著差异：大脑皮质显示广泛而密集的标记，内嗅皮质的标记强度大大超过成年小鼠，丘脑前内侧核有明显标记。
