Departamento de Microbiologia/BIOAGRO, Universidade Federal de Viçosa, MG, Brazil.
J Appl Microbiol. 2008 Nov;105(5):1595-603. doi: 10.1111/j.1365-2672.2008.03905.x.
To study the regulation of the plg1 and plg2 genes of Penicillium griseoroseum, in order to identify the industrial potential of their products in alternative carbon sources that are cheaper and widely available in Brazil.
RT-PCR and Northern blot were used to investigate if plg1 and plg2 expression is under influence of catabolic repression, ambient pH and cAMP. Results demonstrated that the genes were differentially regulated depending on the carbon sources in the culture medium and pH. Sucrose, a noninducing carbon source of the pectinolytic system, was able to promote plg1 transcription but only when yeast extract was added into the culture medium.
The plg genes are differentially expressed. The plg1 gene is more attractive for industrial use due to its expression in alternative carbon sources like sucrose and yeast extract.
In recent years, industries have been trying to replace the toxic conventional treatments employed in these processes by more eco-friendly enzyme treatment. Alternative carbon sources will be tested with the aim to reduce the costs associated to pectin lyase production in Brazil.
研究玫瑰青霉 plg1 和 plg2 基因的调控，以确定其产物在巴西更廉价且广泛可得的替代碳源中的工业潜力。
采用 RT-PCR 和 Northern blot 技术研究 plg1 和 plg2 表达是否受分解代谢阻遏、环境 pH 值和 cAMP 的影响。结果表明，基因的表达根据培养基中的碳源和 pH 值而有所不同。蔗糖是果胶酶系统的非诱导性碳源，能够促进 plg1 转录，但只有在培养基中添加酵母提取物时才会发生。
plg 基因的表达存在差异。plg1 基因因其在蔗糖和酵母提取物等替代碳源中的表达而更具工业应用吸引力。
近年来，各行业一直试图用更环保的酶处理方法替代这些过程中使用的有毒常规处理方法。将对替代碳源进行测试，以期降低巴西果胶酶生产相关成本。
