金脉通胶囊对糖尿病大鼠神经生长因子的影响

[Effects of Jinmaitong capsule on nerve growth factor in diabetic rats].

作者信息

Qu Ling, Liang Xiao-Chun, Wu Qun-Li, Sun Lian-Qin, Zhao Li, Chen Xi-Ping, Zhong Ding-Rong

机构信息

Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2008 Nov;33(21):2539-44.

DOI:

PMID:19149268

Abstract

OBJECTIVE

To study the effects of Jinmaitong Capsule (JMT) on the expression of NGF and NGF mRNA in STZ-induced diabetic rats.

METHOD

Fifty SZT-induced diabetic rats were randomly divided into 5 groups including model group, low-dose JMT group (treated with JMT similar to the quintupling dose of adult recommended dosage), middle-dose JMT group (treated with JMT similar to the decuple dose of adult recommended dosage), high-dose JMT group (treated with JMT similar to the twenty-fold dose of adult recommended dosage) and Neurotropin group (treated with Neurotropin similar to the decuple dose of adult recommended dosage). Ten normal rats matching with weight and age served as normal control group. All rats were given intragastric administration for 16 weeks and then killed. Body weight and blood glucose were detected before and at the 4, 8, 12, 16th week after treatment. The hydrothermal tail-flick and pain threshold to mechanical stimulation with Von Frey filament were carried out before death. The expression of NGF and NGF-mRNA in sciatic nerve were detected by SABC immunohistochemical method and real-time fluorogenetic quantitative PCR respectively.

RESULT

The blood glucose levels of STZ-DM rats were much higher than those of normal rats (P < 0.01). In all the treated groups, there were no significant differences among them compared each other or compared with model group. And it got the same result when concerning about body weight no matter how the rats were dealt with. Hydrothermal tail-flick test: The tail-flick latency of STZ-DM rats were much longer than those of normal rats (P < 0.01 or P < 0.05). Compared with model group, the time shortened significantly in low, middle-dose of JMT groups and Neutrophin group. Compared with normal group, the pain thresholds of model group decreased extremely (P < 0.01). Compared with model group, the threshold values of low-dose, middle-dose JMT group and neutrophin group raised strikingly (P < 0.05). The levels of NGF-mRNA expression in STZ-DM rats were much lower than those of the normal rats (P < 0.01). Compared with model group, NGF-mRNA expression of middle-dose JMT group and Neurotropin group upregulated noticeably (P < 0.01). The integrated option density of NGF expression in STZ-DM rats was much lower than the normal (P < 0.01 or P < 0.05). And the levels of NGF in all the treated groups increased notably compared with model group (P < 0.05 or P < 0.01). There were no significant differences among middle-dose JMT group and Neutrophin group.

CONCLUSION

Traditional Chinese medicine JMT could up-regulate the expression of NGF and NGF-mRNA in sciatic nerve.

摘要

目的

研究金马通胶囊（JMT）对链脲佐菌素（STZ）诱导的糖尿病大鼠神经生长因子（NGF）及其mRNA表达的影响。

方法

将50只STZ诱导的糖尿病大鼠随机分为5组，即模型组、JMT低剂量组（给予相当于成人推荐剂量5倍的JMT）、JMT中剂量组（给予相当于成人推荐剂量10倍的JMT）、JMT高剂量组（给予相当于成人推荐剂量20倍的JMT）和神经妥乐平组（给予相当于成人推荐剂量10倍的神经妥乐平）。选取10只体重、年龄匹配的正常大鼠作为正常对照组。所有大鼠均灌胃给药16周后处死。于治疗前及治疗后第4、8、12、16周检测体重和血糖。处死前进行热板甩尾试验及用von Frey细丝进行机械刺激的痛阈测定。分别采用SABC免疫组化法和实时荧光定量PCR法检测坐骨神经中NGF及其mRNA的表达。

结果

STZ诱导的糖尿病大鼠血糖水平显著高于正常大鼠（P<0.01）。各治疗组之间及与模型组比较，差异均无统计学意义。无论大鼠如何处理，体重方面结果相同。热板甩尾试验：STZ诱导的糖尿病大鼠甩尾潜伏期显著长于正常大鼠（P<0.01或P<0.05）。与模型组比较，JMT低、中剂量组及神经妥乐平组甩尾潜伏期明显缩短。与正常组比较，模型组痛阈极显著降低（P<0.01）。与模型组比较，JMT低、中剂量组及神经妥乐平组痛阈显著升高（P<0.05）。STZ诱导的糖尿病大鼠坐骨神经中NGF-mRNA表达水平显著低于正常大鼠（P<0.01）。与模型组比较，JMT中剂量组及神经妥乐平组NGF-mRNA表达明显上调（P<0.01）。STZ诱导的糖尿病大鼠坐骨神经中NGF表达的积分光密度显著低于正常大鼠（P<0.01或P<0.05）。各治疗组NGF水平与模型组比较均显著升高（P<0.05或P<0.01）。JMT中剂量组与神经妥乐平组之间差异无统计学意义。