Tong Yat-Ching, Cheng Juei-Tang
Department of Urology, School of Medicine, National Cheng Kung University, Tainan, Taiwan, ROC.
BJU Int. 2005 Dec;96(9):1392-6. doi: 10.1111/j.1464-410X.2005.05854.x.
To assess changes associated with diabetes in urinary bladder nerve-growth factor (NGF) and NGF receptor p75NTR expression using a streptozotocin (STZ)-induced diabetic rat model.
Male Wistar rats (32) were divided into four equal groups: group I, vehicle-treated normal rats; group II, vehicle-treated 9-week STZ-diabetic rats; group III, insulin-treated 9-week STZ-diabetic rats; group IV, phlorizin-treated 9-week STZ-diabetic rats. Bladder NGF levels were measured by enzyme-linked immunosorbent assay. Expressions of the mRNAs that encoded NGF and NGF receptor p75NTR in the rat bladder were evaluated using reverse transcription-polymerase chain reaction.
The mean (SEM) NGF level in the STZ-diabetic rat bladder was significantly lower than in the control group, at 56.56 (8.28) and 84.33 (11.05) pg/microg protein, respectively (P < 0.01). The mRNA expressions of bladder NGF and p75NTR in the diabetic rats were significantly lower than in the control group (P < 0.05 and <0.001, respectively). Treatment with either insulin or phlorizin restored the normal blood sugar level; moreover, the bladder NGF levels and the expressions of both NGF and p75NTR mRNAs were normal.
The decrease in the genetic expression of NGF and p75NTR might be responsible for the pathogenesis of diabetic cystopathy while hyperglycaemia is part of the cause of these changes.
使用链脲佐菌素（STZ）诱导的糖尿病大鼠模型，评估膀胱神经生长因子（NGF）和NGF受体p75NTR表达与糖尿病相关的变化。
将32只雄性Wistar大鼠分成四组，每组数量相等：第一组，用赋形剂处理的正常大鼠；第二组，用赋形剂处理的9周龄STZ诱导的糖尿病大鼠；第三组，用胰岛素治疗的9周龄STZ诱导的糖尿病大鼠；第四组，用根皮苷治疗的9周龄STZ诱导的糖尿病大鼠。通过酶联免疫吸附测定法测量膀胱NGF水平。使用逆转录-聚合酶链反应评估大鼠膀胱中编码NGF和NGF受体p75NTR的mRNA表达。
STZ诱导的糖尿病大鼠膀胱中的平均（SEM）NGF水平显著低于对照组，分别为56.56（8.28）和84.33（11.05）pg/μg蛋白（P<0.01）。糖尿病大鼠膀胱NGF和p75NTR的mRNA表达显著低于对照组（分别为P<0.05和<0.001）。用胰岛素或根皮苷治疗可恢复正常血糖水平；此外，膀胱NGF水平以及NGF和p75NTR mRNA的表达均正常。
NGF和p75NTR基因表达的降低可能是糖尿病膀胱病发病机制的原因，而高血糖是这些变化的部分原因。
