Hatzipanagiotou A, Lindner A, Sommer H
Institut für Anatomie, Physiologie und Hygiene, Haustiere der Universität Bonn.
Dtsch Tierarztl Wochenschr. 1991 Jul;98(7):284-6.
The distribution of LDH and CK isoenzymes in blood plasma of ten clinically sound Thoroughbreds with reasonable performance and without elevated clinico-chemical blood variables (reference group) was compared with 57 Thoroughbreds, which had histories of mild locomotor disturbances and/or poor performance and had elevated CK, LDH and/or AST activities (trial group). The trial group was subdivided according to the number of altered blood variables and in the groups with two as well as three altered blood variables also according to the extent of alteration of the total CK activity. The pattern of LDH and CK isoenzyme distribution in the blood plasma of the reference group was the following: 22% LDH1, 36% LDH2, 34% LDH3, 6% LDH4 and 2% LDH5 as well as 75% CK1 and 15% CK2. The remaining 10% of the plasma electropherogram could not be alloted to any one of the two CK bands. All trial groups built showed a similar pattern of changes in their isoenzyme distribution independent on kind and combination of altered enzyme activities. The shares of CK1, LDH4 and LDH5 were significantly increased whilst the shares of CK2, LDH1 and LDH2 decreased. A multiple analysis of variance demonstrated that only increased total CK activities had a pronounced effect on distribution of LDH and CK isoenzyme patterns in the trial group (p less than 0.01 for LDH2, LDH3, LDH4, CK1 and p less than 0.05 for CK2). The conclusion of the study was that the altered distribution pattern of LDH and CK isoenzymes of the trial group signalized an increased skeletal muscle membrane leakage.
将10匹临床健康、表现良好且临床化学血液指标未升高的纯种马（参照组）血浆中乳酸脱氢酶（LDH）和肌酸激酶（CK）同工酶的分布情况，与57匹有轻度运动障碍病史和/或表现不佳且CK、LDH和/或天冬氨酸转氨酶（AST）活性升高的纯种马（试验组）进行比较。试验组根据血液指标改变的数量进行细分，对于血液指标有两项及三项改变的组，还根据总CK活性的改变程度进行细分。参照组血浆中LDH和CK同工酶的分布模式如下：LDH1占22%，LDH2占36%，LDH3占34%，LDH4占6%，LDH5占2%；CK1占75%，CK2占15%。血浆电泳图中其余10%无法归入两条CK条带中的任何一条。所有试验组在同工酶分布上均呈现出相似的变化模式，与酶活性改变的类型和组合无关。CK1、LDH4和LDH5的比例显著增加，而CK2、LDH1和LDH2的比例下降。多因素方差分析表明，在试验组中，仅总CK活性升高对LDH和CK同工酶模式的分布有显著影响（LDH2、LDH3、LDH4、CK1的p值小于0.01，CK2的p值小于0.05）。该研究的结论是，试验组中LDH和CK同工酶分布模式的改变表明骨骼肌膜渗漏增加。
