Goodman H M, Tai L R, Ray J, Cooke N E, Liebhaber S A
Department of Physiology, University of Massachusetts Medical School, Worcester 01655.
Endocrinology. 1991 Oct;129(4):1779-83. doi: 10.1210/endo-129-4-1779.
Genes for normal human pituitary GH (hGH-N) and the GH variant (hGH-V) were expressed in stably transfected mouse mammary cells. The biological properties of hGH-N and hGH-V secreted into the medium were examined using rat adipocytes or epididymal fat segments. Methionyl-hGH produced in E. coli served as a reference standard. The three preparations were quite similar in their ability to bind specifically to intact fat cells and were virtually indistinguishable in their ability to increase glucose oxidation (an insulin-like response), induce refractoriness to insulin-like stimulation, and induce lipolysis in the presence of glucocorticoid. We conclude that placentally expressed hGH-V has a spectrum of metabolic activity comparable to pituitary hGH-N and may contribute to regulation of carbohydrate and lipid metabolism during pregnancy.
正常人垂体生长激素（hGH-N）和生长激素变体（hGH-V）基因在稳定转染的小鼠乳腺细胞中表达。使用大鼠脂肪细胞或附睾脂肪段检测分泌到培养基中的hGH-N和hGH-V的生物学特性。大肠杆菌中产生的甲硫氨酰-hGH用作参考标准。这三种制剂在与完整脂肪细胞特异性结合的能力方面非常相似，在增加葡萄糖氧化（胰岛素样反应）、诱导对胰岛素样刺激的不应性以及在糖皮质激素存在下诱导脂解的能力方面几乎无法区分。我们得出结论，胎盘表达的hGH-V具有与垂体hGH-N相当的一系列代谢活性，可能有助于孕期碳水化合物和脂质代谢的调节。
