Lara H E, Dees W L, Hiney J K, Dissen G A, Rivier C, Ojeda S R
Division of Neuroscience, Oregon Regional Primate Research Center, Beaverton 97006.
Endocrinology. 1991 Oct;129(4):1849-60. doi: 10.1210/endo-129-4-1849.
These experiments were undertaken to define the neuroendocrine mechanisms underlying the recovery of ovarian function after transplantation to an ectopic site. Both ovaries from 23-day-old rats were transplanted to the region of the neck, next to the jugular vein. Serum gonadotropin and plasma immunoreactive inhibin-alpha levels were determined at several intervals thereafter. Serum estradiol (E2) was measured during the first week posttransplantation. Reinnervation of the ovary by sympathetic and sensory nerves was monitored by immunohistochemistry. Sympathetic nerves were identified as adrenergic by the presence of tyrosine hydroxylase (TH), the rate-limiting enzyme in catecholamine biosynthesis, and as peptidergic, by their neuropeptide-Y (NPY) or vasoactive intestinal peptide (VIP) immunoreactivity. Sensory nerves were identified by the presence of substance P (SP) and calcitonin-gene related peptide (CGRP) immunoreactivity. Serum LH and FSH increased, and plasma inhibin levels decreased, within 48 h after transplantation. Serum LH reached maximum levels on day 4, decreasing rapidly thereafter to basal values by day 6. These changes were functionally correlated with the posttransplantation fluctuations in serum E2, which decreased at 48 h, rebounded by day 4, and returned to basal values on day 7. Removal of the transplanted ovaries on day 3 resulted in the disappearance of serum E2 levels on day 4, thus confirming the ovarian graft as the source of E2. In contrast to LH, serum FSH remained significantly elevated for at least 3 weeks after transplantation, then decreased to basal levels after day 21, coinciding with the rise in inhibin secretion. Although a substantial loss of follicles was noted 48 h after transplantation, quantitative examination of the changes on day 4 revealed that approximately 40% of antral follicles were not necrotic. Ovulation and formation of corpora lutea were noted 21 days after transplantation. Reinnervation of the transplanted ovary by TH-, VIP-, NPY-, SP-, and CGRP-containing fibers was first detected 7 days after transplantation. Although VIP reinnervation was sparse and only transiently detected (days 7-21), the density of sympathetic (TH, NPY) and sensory (SP, CGRP) fibers increased 2- to 3-fold between days 7-28, remaining unchanged thereafter. Since apparent completion of this reinnervation coincided with reestablishment of normal levels of both LH and FSH, an additional experiment was performed to determine if the two events were causally related.(ABSTRACT TRUNCATED AT 400 WORDS)
进行这些实验是为了确定异位移植后卵巢功能恢复所依据的神经内分泌机制。将23日龄大鼠的双侧卵巢移植到颈部靠近颈静脉的区域。此后在几个时间点测定血清促性腺激素和血浆免疫反应性抑制素α水平。在移植后的第一周测定血清雌二醇（E2）。通过免疫组织化学监测交感神经和感觉神经对卵巢的再支配。交感神经通过儿茶酚胺生物合成中的限速酶酪氨酸羟化酶（TH）的存在而被鉴定为肾上腺素能神经，并通过其神经肽Y（NPY）或血管活性肠肽（VIP）免疫反应性被鉴定为肽能神经。感觉神经通过P物质（SP）和降钙素基因相关肽（CGRP）免疫反应性的存在而被鉴定。移植后48小时内，血清促黄体生成素（LH）和促卵泡生成素（FSH）升高，血浆抑制素水平降低。血清LH在第4天达到最高水平，此后迅速下降，到第6天降至基础值。这些变化在功能上与移植后血清E2的波动相关，E2在48小时时下降，第4天反弹，第7天恢复到基础值。在第3天切除移植的卵巢导致第4天血清E2水平消失，从而证实卵巢移植物是E2的来源。与LH不同，血清FSH在移植后至少3周内仍显著升高，然后在第21天后降至基础水平，这与抑制素分泌的增加相一致。尽管移植后48小时观察到大量卵泡丢失，但对第4天变化的定量检查显示，约40%的窦状卵泡未坏死。移植后21天观察到排卵和黄体形成。含TH、VIP、NPY、SP和CGRP的纤维对移植卵巢的再支配在移植后7天首次检测到。尽管VIP再支配稀疏且仅在第7 - 21天短暂检测到，但交感神经（TH、NPY）和感觉神经（SP、CGRP）纤维的密度在第7 - 28天增加了2至3倍，此后保持不变。由于这种再支配的明显完成与LH和FSH正常水平的重新建立同时发生，因此进行了另一项实验以确定这两个事件是否存在因果关系。（摘要截短于400字）
