Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Science Park, 510663 Guangzhou, China.
Mol Biol Rep. 2009 Nov;36(8):2323-7. doi: 10.1007/s11033-009-9451-4. Epub 2009 Jan 17.
Macroautophagy is a regulated bulk degradation process of cellular components, mainly long-lived proteins or cytoplasmic organelles. Nutrient depletion is a classic inducer of macroautophagy. In this report, we have induced heat-mediated macroautophagy in several cell lines in the absence of nutrient depletion. Heat treatment increased the autophagic markers LC3-I and LC3-II at the protein levels. Interestingly, expression of a constitutively active HSF1 mutant suppressed basal LC3-II protein level and heat-induced increase of LC3-II. Our results provide evidence that heat is a potent inducer of macroautophagy in mammalian cells, and implicate the negative role of active HSF1 in this process.
自噬是一种细胞成分的受调控的批量降解过程，主要是长寿命蛋白质或细胞质细胞器。营养缺乏是自噬的经典诱导剂。在本报告中，我们在没有营养缺乏的情况下，在几种细胞系中诱导了热介导的自噬。热处理增加了蛋白质水平的自噬标志物 LC3-I 和 LC3-II。有趣的是，组成型激活的 HSF1 突变体的表达抑制了基础 LC3-II 蛋白水平和热诱导的 LC3-II 的增加。我们的结果提供了证据表明，热是哺乳动物细胞中自噬的有效诱导剂，并暗示了活跃的 HSF1 在该过程中的负作用。
