使用UAU1盒式结构进行大规模基因破坏。
Large-scale gene disruption using the UAU1 cassette.
作者信息
Nobile Clarissa J, Mitchell Aaron P
机构信息
Department of Microbiology Columbia University, NY, USA.
出版信息
Methods Mol Biol. 2009;499:175-94. doi: 10.1007/978-1-60327-151-6_17.
Abstract
Candida albicans is a major fungal systemic pathogen in humans. Genetic manipulation of C. albicans is unwieldy. We report here a strategy that is useful and successful for large-scale genetic manipulation of C. albicans genes of interest: use of the UAU1 cassette on a Tn7 transposon. Streamlined yet admittedly flawed disruption techniques, such as the one described here, may prove vital to uncovering the genetic basis of fungal virulence.
摘要
白色念珠菌是人类主要的真菌性全身病原体。对白色念珠菌进行基因操作很困难。我们在此报告一种对感兴趣的白色念珠菌基因进行大规模基因操作有用且成功的策略：在Tn7转座子上使用UAU1盒。像本文所述的这种精简但不可否认有缺陷的破坏技术，可能对揭示真菌毒力的遗传基础至关重要。
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