麻疯树种子萌发和萌发后发育过程中油脂动员的蛋白质组学分析。

Proteomic analysis of oil mobilization in seed germination and postgermination development of Jatropha curcas.

作者信息

Yang Ming-Feng, Liu Yu-Jun, Liu Yun, Chen Hui, Chen Fan, Shen Shi-Hua

机构信息

Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, P. R. China and Institute of Genetics and Developmental Biology, The Chinese Academy of Sciences, Beijing 100080, P. R. China.

出版信息

J Proteome Res. 2009 Mar;8(3):1441-51. doi: 10.1021/pr800799s.

DOI:10.1021/pr800799s

PMID:19152324

Abstract

To understand oil mobilization in germinating seeds, we performed ultrastructural observation and proteomic analysis of endosperm in germinating Jatropha curcas seeds. Results showed that the oil mobilization was initiated during germination, and then the oil was consumed for early seedling development. The significant change in abundance of 50 protein spots during germination indicated that several pathways including beta-oxidation, glyoxylate cycle, glycolysis, citric acid cycle, gluconeogenesis, and pentose phosphate pathway were involved in the oil mobilization.

摘要

为了解发芽种子中的油脂动员情况，我们对麻疯树发芽种子的胚乳进行了超微结构观察和蛋白质组分析。结果表明，油脂动员在种子萌发过程中开始，随后油脂被用于幼苗的早期发育。萌发过程中50个蛋白质点丰度的显著变化表明，包括β-氧化、乙醛酸循环、糖酵解、柠檬酸循环、糖异生和磷酸戊糖途径在内的几种途径参与了油脂动员。