Key Laboratory of Plant Germplasm Enhancement and Speciality Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, P. R. China.
Proteomics. 2011 Jul;11(13):2693-713. doi: 10.1002/pmic.201000598. Epub 2011 Jun 1.
Construction of metabolic and regulatory pathways from proteomic data can contextualize the large-scale data within the overall physiological scheme of an organism. It is an efficient way to predict metabolic phenotype or regulatory style. We did protein profiling in the germinating rice seeds through 1-DE via LC MS/MS proteomic shotgun strategy. In total, 673 proteins were identified, and could be sorted into 14 functional groups. The largest group was metabolism related. The metabolic proteins were integrated into different metabolic pathways to show the style of reserves mobilization and precursor preparation during the germination. Analysis of the regulatory proteins indicated that regulation of redox homeostasis and gene expression also play important roles for the rice seed germination. Although transcription is unnecessary for the germination, it could ensure the rapidity and uniformity of germination. On the contrary, translation with the stored mRNA is required for the germination. This study will help us to further understand the metabolic style, regulation of redox homeostasis, and gene expression during rice seed germination.
从蛋白质组学数据构建代谢和调控途径可以将大规模数据置于生物体的整体生理方案中。这是预测代谢表型或调控方式的有效方法。我们通过 LC MS/MS 蛋白质组学鸟枪法 1-DE 对发芽水稻种子进行了蛋白质谱分析。总共鉴定出 673 种蛋白质，可以分为 14 个功能组。最大的一组与代谢有关。代谢蛋白被整合到不同的代谢途径中，以显示在发芽过程中储备动员和前体准备的方式。调控蛋白的分析表明，氧化还原平衡和基因表达的调控也对水稻种子的萌发起着重要作用。虽然转录对于萌发不是必需的，但它可以确保萌发的快速性和一致性。相反，翻译储存的 mRNA 对于萌发是必需的。这项研究将帮助我们进一步了解水稻种子萌发过程中的代谢方式、氧化还原平衡的调控和基因表达。
