Craik D, Munro S, Nielsen K, Shehan P, Tregear G, Wade J
School of Pharmaceutical Chemistry, Victorian College of Pharmacy Ltd., Parkville, Victoria, Australia.
Eur J Biochem. 1991 Oct 1;201(1):183-90. doi: 10.1111/j.1432-1033.1991.tb16272.x.
Two-dimensional (2D) 1H-NMR spectra of porcine-brain natriuretic peptide (pBNP) have been recorded at 300 MHz and 400 MHz. Peak assignments have been made and the combined information from chemical shifts, coupling constants, temperature coefficients and NOEs have been used to determine the conformational properties of pBNP in (C2H3)2SO. Overall the peptide appears to be flexible, with the possibility of some beta-type structure near the C terminus. Some of the assignments and deduced structural features in the current study differ from those in a recent report by Inooka et al. [Inooka, H., Kikuchi, T., Endo, S., Ishibashi, Y., Wakimasu, M. and Mizuta, E. (1990) Eur. J. Biochem. 193, 127-134] which may indicate the sensitivity of the structure of this peptide to differences in solution conditions.
已在300兆赫和400兆赫下记录了猪脑钠肽（pBNP）的二维（2D）1H-NMR谱。已进行了峰归属，并利用化学位移、耦合常数、温度系数和核Overhauser效应（NOE）的综合信息来确定pBNP在（C2H3）2SO中的构象性质。总体而言，该肽似乎具有柔性，在C末端附近可能存在一些β型结构。本研究中的一些归属和推导的结构特征与Inooka等人最近的报告[Inooka, H., Kikuchi, T., Endo, S., Ishibashi, Y., Wakimasu, M. and Mizuta, E. (1990) Eur. J. Biochem. 193, 127 - 134]中的不同，这可能表明该肽的结构对溶液条件差异的敏感性。
