Williams B B, Bailey E J, Lerner A M
J Lab Clin Med. 1977 Apr;89(4):687-91.
Minimum inhibitory concentrations of 9-beta-D-arabinofuranosyladenine (ara-A, adenine arabinoside, vidarabine) and a purified preparation of 9-beta-D-arabinofuranosylhypoxanthine (arabinoslhypoxanthine, ara-Hx) at end points of 50% MIC50) and 100% (MIC100) reduction to challenges of approximately 50 p.f.u. of herpes simplex virus, type 1 (HSV-1) were determined in vero renal tissue cultures. Adenosine deaminase is universally present in tissue cultures and serum. These same tests were repeated in the presence of a potent inhibitor of adenosine deaminase, R-3-(2-deoxy-beta-D-erythro-pentofuranosyl)-3,6,7,8-tetrahydroimidazo-4,5-d)-(1,3)-diazepin-8-ol (co-vidarabine, co-ara-A). Addition of co-ara-A to assays of MIC50 or MIC100 for ara-A ensures standard reproducible results which can be compared in different laboratories. After incubations of HSV-1 in infected cultures for 96 hours, 35 degrees C., with concentrations of ara-A or ara-Hx at the MIC100 and over, cells were scraped and sonicated. Supernates were then reinoculated into vero flasks free of antiviral agents to determine minimum lethal concentrations (MLC's). Standard values (microng/ml.) for ara-A with co-ara-A are 11.3 (MIC50), 17.0 (MIC100), and 34.0 (MLC) but are 68.1 (MIC50), 170.4 (MIC100) and 375 (MLC) for ara-Hx. These data confirm that as a virustatic agent (MIC100) ara-A is 10 times more active than ara-Hx. Ara-A and ara-Hx have virucidal potentials which require approximately two times the respective MIC100.
在非洲绿猴肾细胞组织培养中，测定了9-β-D-阿拉伯呋喃糖基腺嘌呤（ara-A，阿糖腺苷，阿糖腺嘌呤）和纯化的9-β-D-阿拉伯呋喃糖基次黄嘌呤制剂（阿糖次黄嘌呤，ara-Hx）对大约50个空斑形成单位的1型单纯疱疹病毒（HSV-1）的50%（MIC50）和100%（MIC100）终点抑制浓度。腺苷脱氨酶普遍存在于组织培养物和血清中。在腺苷脱氨酶的强效抑制剂R-3-(2-脱氧-β-D-赤藓戊呋喃糖基)-3,6,7,8-四氢咪唑并[4,5-d]-[1,3]-二氮杂卓-8-醇（环磷腺苷，co-ara-A）存在的情况下重复了这些相同的试验。在ara-A的MIC50或MIC100测定中加入co-ara-A可确保获得可在不同实验室进行比较的标准可重复结果。在35℃下将HSV-1在感染的培养物中培养96小时，使用MIC100及以上浓度的ara-A或ara-Hx，然后刮下细胞并进行超声处理。然后将上清液重新接种到不含抗病毒剂的非洲绿猴肾细胞培养瓶中，以确定最小致死浓度（MLC）。ara-A与co-ara-A的标准值（微克/毫升）分别为11.3（MIC50）、17.0（MIC100）和34.0（MLC），而ara-Hx的标准值分别为68.1（MIC50）、170.4（MIC100）和375（MLC）。这些数据证实，作为一种病毒抑制剂（MIC100），ara-A的活性比ara-Hx高10倍。ara-A和ara-Hx具有杀病毒潜力，所需浓度约为各自MIC100的两倍。
