Schöneshöfer M, Harendt H
J Clin Chem Clin Biochem. 1977 Feb;15(2):69-75. doi: 10.1515/cclm.1977.15.1-12.69.
A specific radioimmunoassay is described which allows the simultaneous determination of serum corticosterone and deoxycortisol. The method involves extraction with dichloromethane, purification by paper chromatography in a modified Bush-system and quantitation by radioimmunoassay. The normal serum concentration of both steroids were found to be dependent on sex and menstrual cycle. Mean concentrations (+/- S.D.) in males, females (follicular phase) and females (luteal phase) were 4210 +/- 2170 ng/1,2410 + 1480 ng/1 and 4390 +/- 2320 ng/1 for corticosterone and 499 +/- 273 ng/1, 207 +/- 152 ng/1 and 335 +/- 182 ng/1 for deoxycortisol. After adrenal stimulation by corticotropin itself or by insulin induced hypoglycemia, the serum concentrations of corticosterone became significantly higher than those of deoxycortisol. After oral administration of dexamethasone serum concentrations of both steroids were suppressed to levels below the limit of the normal range. One hour after oral metyrapone administration at midnight, serum corticosterone decreased, while serum deoxycortisol increased by a factor of about five. After eight hours serum concentrations of both steroids were increased considerably. Corticosterone attained levels slightly higher than the normal range and deoxycortisol rose to levels which were higher than the normal mean concentrations by a factor of about 500.
本文描述了一种特异性放射免疫测定法，可同时测定血清皮质酮和脱氧皮质醇。该方法包括用二氯甲烷萃取、在改良的布什系统中通过纸色谱法纯化以及通过放射免疫测定法定量。发现这两种类固醇的正常血清浓度取决于性别和月经周期。皮质酮在男性、女性（卵泡期）和女性（黄体期）中的平均浓度（±标准差）分别为4210±2170 ng/1、2410 + 1480 ng/1和4390±2320 ng/1，脱氧皮质醇的平均浓度分别为499±273 ng/1、207±152 ng/1和335±182 ng/1。在用促肾上腺皮质激素本身或胰岛素诱导的低血糖刺激肾上腺后，皮质酮的血清浓度显著高于脱氧皮质醇。口服地塞米松后，两种类固醇的血清浓度均被抑制至正常范围下限以下。午夜口服甲吡酮1小时后，血清皮质酮降低，而血清脱氧皮质醇增加约5倍。8小时后，两种类固醇的血清浓度均显著升高。皮质酮达到略高于正常范围的水平，脱氧皮质醇升至比正常平均浓度高约500倍的水平。
