氧化苦参碱对脓毒症休克大鼠肺组织中核因子-κB及其他细胞因子的影响

[Effect of oxymatrine on NF-kappaB and other cell factors in rats lung tissue with septic shock].

作者信息

Zhang Ming-Hao, Li Gui-Zhong, Xu Hua, Zhang Juan, Cao Jun

机构信息

Department of Pathophysiology, Ningxia Medical College of Base College, Yinchuan 750004, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2008 Oct;33(20):2390-4.

PMID:19157136

Abstract

OBJECTIVE

To explore the effects of oxymatrine (OMT) on NF-kappaB and other cell factors in rat lung tissue with septic shock.

METHOD

Fifty-six male SD rats were randomly divided into 7 groups: sham operation group, OMT control group, model (CLP) group, CLP + OMT high, middle, low-dose group, positive control group. Changes in NF-kappaB (p65) and IkB-alpha activity in the pulmonary tissue were determined by immunohistochemical method, tumour necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) levels in pulmonary tissue were determined by radioimmunoassay.

RESULT

OMT could decrease significantly the NF-kappaB (p65) and IkB-alpha activity in the pulmonary tissue (P < 0.05), TNF-alpha and IL-6 levels in pulmonary tissue homogenate decreased markedly (P < 0.05). OMT could elevate the content of PaO2, SaO2, decrease the content of PaCO2, HCO3- and decrease the ratio between wet weight of the lung and dry weight of the lung and the PWI.

CONCLUSION

OMT can inhibit NF-kappaB-inducing kinase (NIK), NF-kappaB activity and reduce the expression of proinflammatory factor (TNF-alpha, IL-6) and antagonize the lung injury in a rat model of septic shock.

摘要

目的

探讨氧化苦参碱（OMT）对脓毒症休克大鼠肺组织中核因子-κB（NF-κB）及其他细胞因子的影响。

方法

将56只雄性SD大鼠随机分为7组：假手术组、OMT对照组、模型（CLP）组、CLP + OMT高、中、低剂量组、阳性对照组。采用免疫组织化学方法检测肺组织中NF-κB（p65）和IkB-α活性变化，采用放射免疫分析法检测肺组织中肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）水平。

结果

OMT可显著降低肺组织中NF-κB（p65）和IkB-α活性（P < 0.05），肺组织匀浆中TNF-α和IL-6水平明显降低（P < 0.05）。OMT可提高动脉血氧分压（PaO2）、动脉血氧饱和度（SaO2）含量，降低动脉血二氧化碳分压（PaCO2）、碳酸氢根（HCO3-）含量，并降低肺湿重与干重之比及肺灌注指数（PWI）。