Hata H, Kojima S
Department of Parasitology, Chiba University School of Medicine, Japan.
Exp Parasitol. 1991 Oct;73(3):354-61. doi: 10.1016/0014-4894(91)90107-8.
The third-stage larvae of Angiostrongylus costaricensis were successfully cultured to young adults in a chemically defined medium. The most suitable medium for the development was Waymouth's medium among eight defined media examined. Twenty-eight days after cultivation in this medium, 77% of the larvae developed to young adults, although these worms gradually died thereafter. When Waymouth's medium was supplemented with mouse red blood cells, these young adult worms continued their development. The mean body lengths of the worms cultivated in Waymouth's medium supplemented with RBCs were significantly larger than those of the worms in the medium without RBCs on Days 14 and 21 after cultivation. Addition of RBCs was essential for their further development. At 28 days after cultivation, the maximum body length of the worms was 2.1 mm for males and 3.3 mm for females. Additions of serum, yeast extract lactalbumin hydrolysate, and growth factors to Waymouth's medium did not provide any additional benefits for worm development.
哥斯达黎加管圆线虫的三期幼虫在化学成分明确的培养基中成功培养至成虫早期阶段。在所检测的八种确定培养基中，最适合其发育的是韦茅斯培养基。在该培养基中培养28天后，77%的幼虫发育至成虫早期阶段，不过这些虫体此后逐渐死亡。当韦茅斯培养基中添加小鼠红细胞时，这些成虫早期阶段的虫体能够继续发育。在添加红细胞的韦茅斯培养基中培养的虫体，培养后第14天和第21天的平均体长显著大于未添加红细胞培养基中的虫体。添加红细胞对其进一步发育至关重要。培养28天后，雄虫的最大体长为2.1毫米，雌虫为3.3毫米。在韦茅斯培养基中添加血清、酵母提取物、水解乳白蛋白和生长因子对虫体发育并无额外益处。
