Provost P R, Marcel Y L, Milne R W, Weech P K, Rassart E
Département des sciences biologiques, Université du Québec à Montréal, Canada.
FEBS Lett. 1991 Sep 23;290(1-2):139-41. doi: 10.1016/0014-5793(91)81244-3.
We studied apolipoprotein D (apoD) mRNA in primary cultures of human diploid fibroblasts (HDF). In early-passage HDF no apoD mRNA was detected in replicating cells in sparse culture, but the gene was expressed in quiescent cells in confluent and in serum-starved cultures. In contrast, late-passage HDF expressed apoD mRNA in sparse culture, but the level increased after attainment of confluence. Thus fibroblasts, the common cell-type expressing apoD mRNA in vivo, express this characteristic following growth-arrest. The same pattern of activation was found in another fibroblast cell line deficient in apoB/E (LDL) receptors, excluding a role for cellular cholesterol delivery by the LDL-receptor pathway controlling apoD expression.
我们研究了人二倍体成纤维细胞（HDF）原代培养物中的载脂蛋白D（apoD）mRNA。在早期传代的HDF中，稀疏培养的增殖细胞中未检测到apoD mRNA，但该基因在汇合培养和血清饥饿培养的静止细胞中表达。相比之下，晚期传代的HDF在稀疏培养中表达apoD mRNA，但汇合后水平升高。因此，成纤维细胞是体内表达apoD mRNA的常见细胞类型，在生长停滞时表现出这种特性。在另一种缺乏apoB/E（低密度脂蛋白）受体的成纤维细胞系中也发现了相同的激活模式，排除了通过低密度脂蛋白受体途径进行细胞胆固醇传递来控制apoD表达的作用。
