Kettunen R, Tyystjärvi E, Aro E M
Department of Biology, University of Turku, Finland.
FEBS Lett. 1991 Sep 23;290(1-2):153-6. doi: 10.1016/0014-5793(91)81247-6.
Illumination of intact pumpkin leaves with high light led to severe photoinhibition of photosystem II with no net degradation of the D1 protein. Instead, however, a modified form of D1 protein with slightly slower electrophoretic mobility was induced with corresponding loss in the original form of the D1 protein. When the leaves were illuminated in the presence of chloramphenicol the modified form was degraded, which led to a decrease in the total amount of the D1 protein. Subfractionation of the thylakoid membranes further supported the conclusion that the novel form of the D1 protein was not a precursor but a high-light modified form that was subsequently degraded.
用强光照射完整的南瓜叶片会导致光系统II严重光抑制，而D1蛋白没有净降解。然而，相反地，诱导出了一种电泳迁移率略慢的D1蛋白修饰形式，同时原始形式的D1蛋白相应减少。当叶片在氯霉素存在下照射时，修饰形式被降解，这导致D1蛋白总量减少。类囊体膜的亚分级进一步支持了以下结论：D1蛋白的新形式不是前体，而是随后被降解的高光修饰形式。
