Yasui Takeshi, Takahashi Yu, Fukushima Shuichiro, Ogura Yuki, Yamashita Toyonobu, Kuwahara Tomohiro, Hirao Tetsuji, Araki Tsutomu
Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan.
Opt Express. 2009 Jan 19;17(2):912-23. doi: 10.1364/oe.17.000912.
Optical probe methods for in vivo assessments of cutaneous photoaging are necessary in fields such as anti-aging dermatology and skin cosmetic development. We investigated the relation between wrinkle direction and collagen orientation in ultraviolet-B-exposed (UVB-exposed) skin using polarization-resolved second-harmonic-generation (SHG) microscopy. A polarization anisotropic image of the SHG light indicated that wrinkle direction in UVB-exposed skin is predominantly parallel to the orientation of dermal collagen fibers. Furthermore, collagen orientation in post-UVB-exposed skin with few wrinkles changed from that of UVB-exposed wrinkled skin to that of no-UVB-exposed skin. The method proposed has the potential to become a powerful non-invasive tool for assessment of cutaneous photoaging.
用于皮肤光老化体内评估的光学探针方法在抗衰老皮肤病学和皮肤化妆品开发等领域是必要的。我们使用偏振分辨二次谐波产生（SHG）显微镜研究了紫外线B照射（UVB照射）皮肤中皱纹方向与胶原蛋白取向之间的关系。SHG光的偏振各向异性图像表明，UVB照射皮肤中的皱纹方向主要与真皮胶原纤维的取向平行。此外，皱纹较少的UVB照射后皮肤中的胶原蛋白取向从UVB照射的皱纹皮肤转变为未UVB照射的皮肤。所提出的方法有可能成为评估皮肤光老化的强大非侵入性工具。
