Kerman Kagan, Vestergaard Mun'delanji, Tamiya Eiichi
Department of Chemistry, University of Western Ontario, London, ON, Canada.
Methods Mol Biol. 2009;504:99-113. doi: 10.1007/978-1-60327-569-9_7.
An electrochemical DNA biosensor is a device that utilizes the inherent ability of two complementary strands of nucleic acids to form a double helix. The specificity of this reaction, namely hybridization, is used in the detection of target DNA sequences with a view toward developing point-of-care devices. Since the early 1990s, great progress has been made in this field, but there are still numerous challenges to overcome. This chapter describes the components of an electrochemical DNA biosensor for researchers new to the field, paying particular attention to intercalator-based DNA biosensors. We will use a well-defined electro-active DNA intercalator Hoechst 33258, as our running example. Two of the most classic DNA sensing methods: solution-based and surface-immobilized methods are discussed, along with guiding notes that would help identify and overcome possible problems in a typical electrochemical DNA biosensor experiment.
电化学DNA生物传感器是一种利用两条互补核酸链形成双螺旋的固有能力的装置。这种反应的特异性，即杂交，被用于检测目标DNA序列，以期开发即时检测设备。自20世纪90年代初以来，该领域已取得了巨大进展，但仍有许多挑战有待克服。本章为该领域的新手研究人员介绍电化学DNA生物传感器的组成部分，特别关注基于嵌入剂的DNA生物传感器。我们将使用一种定义明确的电活性DNA嵌入剂Hoechst 33258作为我们的实例。讨论了两种最经典的DNA传感方法：基于溶液的方法和表面固定方法，以及有助于识别和克服典型电化学DNA生物传感器实验中可能出现的问题的指导说明。
