转酮醇酶样基因TKTL1对人鼻咽癌发生和转移的影响

[Effects of transketolase-like gene TKTL1 on occurrence and metastasis of human nasopharyngeal carcinoma].

作者信息

Zhang Song, Yang Ju-Hong, Cai Peng-Cheng

机构信息

Department of Otolaryngology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2008 Dec 2;88(44):3131-4.

PMID:19159596

Abstract

OBJECTIVE

To investigate the effects of transketolase-like gene TKTL1 on the occurrence and metastasis of human nasopharyngeal carcinoma (NPC).

METHODS

Real-time PCR was used to determine the mRNA expression levels of transketolase gene family (TKT, TKTL1, and TKTL2) in the 65 biopsy specimens of human nasopharyngeal carcinoma, 9 at stage I, 15 at stage II, 16 at stage III, 13 at stage IVA, and 12 at stage IVB, 42 with metastasis and 232 without metastasis, and 9 biopsy specimens of chronic nasopharyngitis.

RESULTS

The TKL activity level of the NPC tissues was 21.6 +/- 2.8, significantly higher than that of the chronic nasopharyngitis tissues (6.2 +/- 1.3, P < 0.01). The TKL activity level of the NPC tissues with metastasis was 26.5 +/- 3.2, significantly higher than that of the NPC tissues without metastasis (17.6 +/- 2.1, P < 0.01). There was no significant difference in the expression levels of TKT and TKTL2 genes between the human NPC and chronic nasopharyngitis tissues (both P > 0.05). The expression level of TKTL1 gene in the NPC tissues was 5.25 +/- 0.32, significantly higher than that in the chronic nasopharyngitis tissues (0.98 +/- 0.11, t = 6.23, P < 0.01), and the expression level of TKTL1 gene in the NPC tissues with lymph node metastasis was 5.86 +/- 0.38, significantly higher than that in the NPC tissues without lymph node metastasis (4.18 +/- 0.22, t = 3.28, P < 0.01).

CONCLUSION

TKTL1 may play an important role in the occurrence and metastasis of human NPC and become a potential target for novel anti-cancer therapy.

摘要

目的

探讨转酮醇酶样基因TKTL1对人鼻咽癌（NPC）发生及转移的影响。

方法

采用实时荧光定量PCR检测65例人鼻咽癌活检标本中转酮醇酶基因家族（TKT、TKTL1和TKTL2）的mRNA表达水平，其中Ⅰ期9例，Ⅱ期15例，Ⅲ期16例，ⅣA期13例，ⅣB期12例，有转移42例，无转移23例，以及9例慢性鼻咽炎活检标本。

结果

NPC组织的TKL活性水平为21.6±2.8，显著高于慢性鼻咽炎组织（6.2±1.3，P<0.01）。有转移的NPC组织的TKL活性水平为26.5±3.2，显著高于无转移的NPC组织（17.6±2.1，P<0.01）。人NPC组织与慢性鼻咽炎组织中TKT和TKTL2基因的表达水平无显著差异（均P>0.05）。NPC组织中TKTL1基因的表达水平为5.25±0.32，显著高于慢性鼻咽炎组织（0.98±0.11，t=6.23，P<0.01），有淋巴结转移的NPC组织中TKTL1基因的表达水平为5.86±0.38，显著高于无淋巴结转移的NPC组织（4.18±0.22，t=3.28，P<0.01）。