Zhang Yue, Shi Heping
College of Life Sciences, South China Normal University, Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, Guangzhou 510631, China.
Sheng Wu Gong Cheng Xue Bao. 2008 Sep;24(9):1604-12.
Effects of phosphorus deficiency in the medium on growth and morphology and activities of SOD and POD, utilization of nitrogen and calcium in the medium during liquid culture of Cucumis sativus hairy roots were investigated. The results showed that C. sativus hairy roots can not grow in the medium without addition of any phosphorus. When cultured into the medium with different Pi concentrations, the growth of C. sativus hairy roots was significantly inhibited with the decreasing of Pi concentration in the medium, its main roots became thinner and longer, the number of its lateral roots was decreased and its lateral roots became shorter and smaller. Compared to the medium with full strength phosphorus, the content of soluble proteins in C. sativus hairy roots cultured under Pi deficiency was significantly lower than that with standard full-length Pi, while POD and SOD activities in C. sativus hairy roots were significantly stimulated. Compared to the control (without addition of any phosphorus in the medium), the activities of POD and SOD in C. sativus hairy roots cultured in the medium with different concentration of Pi were lower than the control. When C. sativus hairy roots were cultured into medium with different Pi concentrations, the medium conductivity was gradually decreased with time and with direct proportion of the initial Pi concentration of the medium; NH4(+)-N and NO3(-)-N of the medium was gradually absorbed and utilized, at day 15, NH4(+)-N of the medium was nearly used up but its NO3(-)-N was not used up until cultured for 30 days. Pi deficiency in the medium could decrease the consumption rate of NO3(-)-N and inhibited the absorption and utilization of calcium of the medium by C. sativus hairy roots. Proper enhancement of Pi concentration could stimulate absorption and consumption of calcium of the medium.
研究了培养基中缺磷对黄瓜毛状根液体培养过程中生长、形态以及超氧化物歧化酶（SOD）和过氧化物酶（POD）活性、培养基中氮和钙利用的影响。结果表明，不添加任何磷的培养基中黄瓜毛状根无法生长。在不同磷浓度的培养基中培养时，随着培养基中磷浓度的降低，黄瓜毛状根的生长受到显著抑制，其主根变细变长，侧根数量减少且侧根变短变小。与全磷培养基相比，缺磷培养的黄瓜毛状根中可溶性蛋白含量显著低于标准全磷培养基，而黄瓜毛状根中的POD和SOD活性显著增强。与对照（培养基中不添加任何磷）相比，在不同磷浓度培养基中培养的黄瓜毛状根中POD和SOD活性低于对照。当黄瓜毛状根在不同磷浓度的培养基中培养时，培养基电导率随时间逐渐降低，且与培养基初始磷浓度成正比；培养基中的NH4(+)-N和NO3(-)-N被逐渐吸收利用，在第15天时，培养基中的NH4(+)-N几乎耗尽，但直到培养30天时其NO3(-)-N才被耗尽。培养基中缺磷会降低NO3(-)-N的消耗速率，并抑制黄瓜毛状根对培养基中钙的吸收和利用。适当提高磷浓度可刺激培养基中钙的吸收和消耗。
