Zhang Hailing, Chai Xiuli, Luo Guoliang, Wang Fengxue, Yi Li, Shao Xiqun, Yan Xijun
Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Jilin 132109, China.
Sheng Wu Gong Cheng Xue Bao. 2008 Sep;24(9):1625-30.
In order to characterize the biological activity of fox (Vulpes vulpes) interferon gamma(VuIFN-gamma), We have isolated the cDNA encoding arctic fox (Alopex lagopus) VuIFN-gamma. This cDNA encodes a 23 amino acid signal peptide and a 144 amino acid mature protein, which shares 99.8% or 99.4% for nucleotide identity with silver fox and canine, respectively, and 100% for amino acid identity. Expression of recombinant mature arctic fox interferon gamma (mVuIFN-gamma) in bacterial system was confirmed by SDS-PAGE and Western blotting analysis. Recombinant VuIFN-gamma showed higher antiviral activity against vesicular stomatitis virus in cultured Vero and MDCK by inhibiting virus induced cytopathic effect, In view of the immunomodulatory and antiviral activities of VuIFN-gamma, it may provide a basis for further research on antiviral therapy of recombinant VuIFN-gamma in economic animal practice.
为了表征狐狸（赤狐）γ干扰素（VuIFN-γ）的生物学活性，我们分离了编码北极狐（Alopex lagopus）VuIFN-γ的cDNA。该cDNA编码一个23个氨基酸的信号肽和一个144个氨基酸的成熟蛋白，其核苷酸序列与银狐和犬的核苷酸同一性分别为99.8%或99.4%，氨基酸同一性为100%。通过SDS-PAGE和Western印迹分析证实了重组成熟北极狐γ干扰素（mVuIFN-γ）在细菌系统中的表达。重组VuIFN-γ通过抑制病毒诱导的细胞病变效应，对培养的Vero细胞和MDCK细胞中的水泡性口炎病毒显示出更高的抗病毒活性。鉴于VuIFN-γ的免疫调节和抗病毒活性，它可能为经济动物实践中重组VuIFN-γ抗病毒治疗的进一步研究提供基础。
