Jin Xiao-Bao, Wang Yan, Zhu Jia-Yong, Ma Yan, Chu Fu-Jiang, Yang Xiao-Rong
Department of Parasitology, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2008 Jun 30;26(3):187-90.
To explore the expression level of antibacterial peptide genes at the different development stages of Musca domestica.
Total RNA was extracted from eggs, 1st instar larvae, 2nd instar larvae, 3rd instar larvae, pupae and adults of M. domestica. After the primers for antibacterial peptide (cecropin, defensin and attacin) genes and GAPDH were designed respectively according to the reported M. domestica gene sequences in GenBank, semi-quantitative RT-PCR was performed to detect expression level of these genes in the development stages of M. domestica using GAPDH as inner control.
The antibacterial peptide genes were detected with bands of 210 bp, 300 bp and 650 bp at all development stages of M. domestica. The expression level in the 3rd instar larvae and adults were higher, with a band value of cecropin, defensin and attacin in relation to GAPDH of 1.61, 1.99, 1.62 and 1.47, 1.92, 1.59, respectively; while it was lower at eggs, 1st instar larvae and pupae with a band value of cecropin, defensin and attacin 0.49, 0.49, 0.42 and 0.72, 0.49, 0.64 and 0.65, 0.39, 0.91, respectively.
The antibacterial peptide genes express at all development stages of M. domestica with an evidently different expression level.
探讨家蝇不同发育阶段抗菌肽基因的表达水平。
提取家蝇卵、1龄幼虫、2龄幼虫、3龄幼虫、蛹和成虫的总RNA。根据GenBank中报道的家蝇基因序列分别设计抗菌肽（天蚕素、防御素和攻击素）基因及GAPDH的引物，以GAPDH为内参，采用半定量RT-PCR检测这些基因在家蝇各发育阶段的表达水平。
在家蝇的所有发育阶段均检测到210 bp、300 bp和650 bp的抗菌肽基因条带。3龄幼虫和成虫中的表达水平较高，天蚕素、防御素和攻击素相对于GAPDH的条带值分别为1.61、1.99、1.62和1.47、1.92、1.59；而在卵、1龄幼虫和蛹中的表达水平较低，天蚕素、防御素和攻击素的条带值分别为0.49、0.49、0.42和0.72、0.49、0.64以及0.65、0.39、0.91。
抗菌肽基因在家蝇的所有发育阶段均有表达，且表达水平存在明显差异。
